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- 2026年04月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 细胞形态：
上皮样
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 运输方式：
冻存运输
- 器官来源：
其他
- ATCC Number：
CCL-25™
- 生长状态：
贴壁生长
| ##Caption## | |||
| Designations: | WISH | ||
| Depositors: | L Hayflick | ||
| Biosafety Level: | 2 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: HeLa contaminant | ||
| Cellular Products: | keratin | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | NOTE: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination The cells are positive for keratin by immunoperoxidase staining. |
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| DNA Profile (STR): | Amelogenin: X CSF1PO: 9,10 D13S317: 13.3 D16S539: 9,10 D5S818: 11,12 D7S820: 8,12 THO1: 7 TPOX: 8,12 vWA: 16,18 |
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| Isoenzymes: | G6PD, A | ||
| HeLa Markers: | Y | ||
| Comments: | This line was originally thought to be derived from normal amnion, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination. The cells are positive for keratin by immunoperoxidase staining. NOTE: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination |
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| Propagation: | ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10% Temperature: 37.0°C |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended Medium Renewal: Twice per week Remove medium, add fresh 0.25% trypsin solution for 3 to 5 minutes, remove trypsin and let culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 |
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| References: | 22153: Hayflick L. The establishment of a line (WISH) of human amnion cells in continuous cultivation. Exp. Cell Res. 23: 14-20, 1961. PubMed: 13712490 26034: . . Am. J. Dis. Child. 103: 460, 1962. |
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文献和实验wish细胞是上皮细胞， 人羊膜细胞 ，培养基我用的是最常见的1640（10％小牛血清，加双抗）。1、细胞长满培养瓶时既要传代。我用胰酶和edta的混合液消化。用D-Hank's液配制成0.25%的胰酶。edta的终浓度是0.02%。2、使用前37度预热，每个25ml培养瓶加9滴的胰酶和edta混合液，消化液的量可根据自己培养瓶的大小而定，原则就是能够盖满瓶底。加入胰酶和edta混合液以后，为使酶的活性最大，将培养瓶盖拧紧后放回培养箱中，5分钟左右即用含血清的培养基终止消化。如果在室温情况下消
Gene expression can be analyzed at high spatial resolution via RNA in situ detection methods. For many tissues and species, these will be performed on sections of embedded and fixed plant material. When very small or fragile tissues
immediately in a TA Cloning® or TOPO TA Cloning® reaction. If you wish to store your reaction, continue to Step 4. 4. Extract reaction immediately with an equal volume of phenol-chloroform. This removes all of the polymerases. 5. Precipitate the DNA
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