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- 详细信息
- 技术资料
- 相关疾病：
正常
- 器官来源：
肾脏
- ATCC Number：
CRL-1569™
- 生长状态：
混合型生长
- 库存：
大量
- 运输方式：
冻存运输
- 物种来源：
大鼠
- 是否是肿瘤细胞：
0
- 细胞类型：
其他细胞类型
| Designations: | KNRK | ||
| Depositors: | JE DeLarco | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | mixed: adherent and suspension | ||
| Organism: | Rattus norvegicus deposited as Rattus sp. | ||
| Morphology: | |
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| Source: | Organ: kidney Disease: normal Cell Type: Kirsten murine sarcoma virus (Ki-MSV) transformed |
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| Cellular Products: | The cells release growth factors in serum free medium. | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | The cells do not produce Ki-MSV particles. Tested and found negative for ectromelia virus (mousepox). |
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| Propagation: | ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 95%; bovine calf serum, 5% |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended Medium Renewal: 2 to 3 times per week Cultures can be maintained by transferring floating cells to additional flasks. Attached cells may be subcultured using 0.25% trypsin-0.03% EDTA. Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks. |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 | ||
| References: | 22501: Aaronson SA, Weaver CA. Characterization of murine sarcoma virus (Kirsten) transformation of mouse and human cells. J. Gen. Virol. 13: 245-252, 1971. PubMed: 4333712 32931: Gimona M, et al. Forced expression of tropomyosin 2 or 3 in v-Ki-ras-transformed fibroblasts results in distinct phenotypic effects. Proc. Natl. Acad. Sci. USA 93: 9618-9623, 1996. PubMed: 8790379 33135: Bohm SK, et al. Mechamisms of desensitization and resensitization of proteinase-activated receptor-2. J. Biol. Chem. 271: 22003-22016, 1996. PubMed: 8703006 |
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