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- 详细信息
- 技术资料
- 运输方式:
冻存运输
- 生长状态:
悬浮生长
- 库存:
大量
- 免疫类型:
IgG2a
- 细胞形态:
淋巴样
- ATCC Number:
CRL-2179™
- 物种来源:
大鼠
- 是否是肿瘤细胞:
0
| Designations: | KMI6 | ||
| Depositors: | PW Kincade | ||
| Isotype: | IgG2a | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against mouse integrin beta 1 subunit (CD29) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | 1. This hybridoma is provided for research purposes only. Neither the cell line nor the antibody derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purpose of sale, or producing for sale, cells or their products. The cells are provided as a service to the research community. They are provided without warranty or merchantability of fitness for a particular purpose or any other warranty, express or implied. 2. Any proposed commercial use of these cells or antibody produced from them must first be negotiated with the Oklahoma Medical Research Foundation, 825 N.E. 13th, Oklahoma City, OK 73104. Telephone (405) 271-7905. | ||
| Comments: | Animals were immunized with BMS2 murine bone marrow stromal cells. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. This line was originally characterized as a murine stromal cell restricted marker. The antigenic specificity has now been identified as the murine integrin beta 1 homolog. Unlike the alpha 4 specific antibody PS/2 (see ATCC CRL-1911 ), KMI6 did not block lymphoid cell adhesion. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| References: | 22810: Wu X, et al. Recognition of murine integrin beta 1 by a rat anti-stromal cell monoclonal antibody. Hybridoma 13: 409-416, 1994. PubMed: 7532155 | ||
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KMI6
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