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- 2026年04月11日
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- 详细信息
- 技术资料
- 免疫类型：
IgG2a
- 细胞形态：
淋巴样
- 物种来源：
大鼠
- 是否是肿瘤细胞：
0
- 库存：
大量
- 生长状态：
悬浮生长
- 运输方式：
冻存运输
- ATCC Number：
CRL-2212™
| Designations: | KMC8.8 | ||
| Depositors: | PW Kincade | ||
| Isotype: | IgG2a | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against mouse CD9 | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | Note: These cells are distributed for research purposes only. The Oklahoma Medical Research Foundation releases the line subject to the following: 1) The KMC8.8 cells or antibody derived from it must not be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purpose of sale, or producing for sale, cells or their product. Commercial interests are the exclusive property of the Oklahoma Medical Research Foundation. 2) Any proposed commercial use of these cells or antibody produced by them must first be negotiated with the Oklahoma Medical Research Foundation, 825 N.E. 13th, Oklahoma City, OK 73104. Telephone (405) 271-7905, FAX (405) 271-8568; 3) In all papers reporting any use of these cells or derived products, a direct reference will be made to the above original publication. | ||
| Tumorigenic: | Yes | ||
| Comments: | Animals were immunized with BMS2 murine bone marrow stromal cells. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with mouse CD9 (expressed by bone marrow stromal cells, megakaryocytes, platelets, myeloid cells and subpopulations of mature lymphocytes. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
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| References: | 22130: . . Blood 87: 1996.. | ||
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KMC8.8
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