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- 细胞形态：
上皮样
- 相关疾病：
其他疾病
- ATCC Number：
CRL-2065™
- 生长状态：
贴壁生长
- 库存：
大量
- 器官来源：
睾丸
- 运输方式：
冻存运输
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 品系：
C57BL/6
- 细胞类型：
其他细胞类型
| Designations: | MLTC-1 | ||
| Depositors: | RV Rebois | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent, Cells form islands that dome and the monolayer may not become confluent. | ||
| Organism: | Mus musculus | ||
| Morphology: | epithelial |
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| Source: | Organ: testis Strain: C57BL/6 Disease: Leydig cell tumor Cell Type: Leydig cell; |
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| Cellular Products: | progesterone [22276 ] | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host (Roche Transfection Reagents ) | ||
| Receptors: | chorionic gonadotropin; luteinizing hormone (LH) [22276 ] | ||
| Tumorigenic: | Yes | ||
| Cytogenetic Analysis: | polyploid; modal number = 95 [22276 ] | ||
| Gender: | male | ||
| Comments: | This line was established from the M548OP transplantable Leydig cell tumor carried in C57BL/6 mice. [22276 ] The cells retain the hormonal responsiveness of the original tumor. Membrane adenyl cyclase activity is stimulated by treatment with human chorionic gonadotropin (hCG), luteinizing hormone, cholera toxin, sodium fluoride and guanyl-5'-ylimidodiphosphate, and produce progesterone in response to hCG. [22276 ] |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: Every 3 to 4 days Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
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| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| Doubling Time: | 35 to 40 hrs | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
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| References: | 22276: Rebois RV. Establishment of gonadotropin-responsive murine leydig tumor cell line. J. Cell Biol. 94: 70-76, 1982. PubMed: 6288740 | ||
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MLTC-1
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