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- 年限：
5 month old
- 库存：
大量
- 细胞形态：
上皮样
- 品系：
FVB/N
- 器官来源：
肺
- 生长状态：
贴壁生长
- 物种来源：
转基因小鼠
- 是否是肿瘤细胞：
0
- 细胞类型：
其他细胞类型
- ATCC Number：
CRL-2110™
- 运输方式：
冻存运输
| Designations: | MLE 12 | ||
| Depositors: | JA Whitsett | ||
| Biosafety Level: | 2 [CELLS CONTAIN PAPOVAVIRUS ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus , transgenic deposited as mouse, transgenic | ||
| Morphology: | epithelial |
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| Source: | Organ: lung Strain: FVB/N Cell Type: epithelialSV40 transformed |
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| Cellular Products: | lung surfactant proteins B and C (SP-B, SP-C) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: 1992 | ||
| Applications: | Lung surfactant proteins B and C were detected. The cells secrete phospholipids in response to phorbol esters and ATP but not in response to forskolin. This line was established in 1992 by Kathryn A. Wikenheiser from pulmonary tumors in a mouse transgenic for the SV40 large T antigen under the control of the promoter region of the human surfactant protein C gene. |
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| Tumorigenic: | Yes | ||
| Age: | 5 month old | ||
| Gender: | female | ||
| Comments: | This line was established in 1992 by Kathryn A. Wikenheiser from pulmonary tumors in a mouse transgenic for the SV40 large T antigen under the control of the promoter region of the human surfactant protein C gene. The cells express the mRNA for large T antigen. Lung surfactant proteins B and C were detected. The cells secrete phospholipids in response to phorbol esters and ATP but not in response to forskolin. |
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| Propagation: | ATCC complete growth medium: HITES medium supplemented with 2% fetal bovine serum
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended Medium Renewal: Twice per week |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Doubling Time: | 7 to 9 hrs | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006 | ||
| References: | 23101: Wikenheiser KA, et al. Simian virus 40 large T antigen directed by transcriptional elements of the human surfactant protein C gene produces pulmonary adenocarcinomas in transgenic mice. Cancer Res. 52: 5342-5352, 1992. PubMed: 1394139 23539: Wikenheiser KA, et al. Production of immortalized distal respiratory epithelial cell lines from surfactant protein C/simian virus 40 large tumor antigen transgenic mice. Proc. Natl. Acad. Sci. USA 90: 11029-11033, 1993. PubMed: 8248207 |
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MLE 12
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