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- 品系：
BALB/c
- 相关疾病：
骨肉瘤
- ATCC Number：
CRL-2837™
- 生长状态：
贴壁生长
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 细胞形态：
其他
- 细胞类型：
其他细胞类型
- 库存：
大量
- 运输方式：
冻存运输
| Designations: | K7M2-pCI Neo | ||
| Depositors: | C Khanna | ||
| Biosafety Level: | 2 [Cells containing CMV and SV40 viral DNA sequences ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus | ||
| Morphology: | osteoblast |
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| Source: | Disease: osteosarcoma Strain: BALB/c Derived from metastatic site: lung Cell Type: osteoblast; |
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| Cellular Products: | decorin [89453 ] secreted phosphoprotein 1 (osteopontin) [89453 ] villin 2 (ezrin) [89454 ] |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: 2004 (The K7M2-pCI Neo cell line was derived from the K7M2 wt (ATCC CRL-2836 ) cell line. K7M2 wt cells were transfected with the pCI Neo vector. The vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells. It also contains SV40 viral sequences and the neomycin resistance gene.) | ||
| Applications: | tumor model | ||
| Tumorigenic: | Yes | ||
| Comments: | Antigen Expression: CD31 + [PubMed:1315100] Products: factor VIII [PubMed: 11315100]; integrin binding sialoprotein (BSP) [PubMed11315100]; biglyan [PubMed: 11315100]; decorrin [PubMed:1315100]; villin 2 (ezrin) [PubMed: 11325848]. Expression of bone sialoprotein, biglyan, decorrin, and osteopontin is suggestive of bone lineage cells. The K7M2 wt cell line was derived by intra-osseous injection of the K7 murine osteosarcoma cell line to the proximal tibia of a BALB/c mouse. The resultant primary tumor spontaneously metastasized to the lungs. A pulmonary metastasis was removed from the lung and then surgically implanted to a paraosteal tibial muscle flap of a na�ve mouse. This cycle of implantation of pulmonary metastasis to paraosteal tibial muscle flap sites was repeated, resulting in the K7M2 primary tumor. This tumor was dissociated to a single cell suspension to create the K7M2 wt cell line [PubMed:11315100]. Expression of bone sialoprotein, biglyan, decorrin, and osteopontin is suggestive of bone lineage cells. |
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| Propagation: | ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 0.8 mg/ml G-418 and 10% fetal bovine serum Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol:
Interval: Maintain cultures at a cell concentration between 3 X 10(4) and 1 X 10(5) cells/sq. cm Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended Medium Renewal: Two to three times weekly |
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| Doubling Time: | 12 hours | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 parental cell line:ATCC CRL-2836 |
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| References: | 89453: Khanna C, et al. An orthotopic model of murine osteosarcoma with clonally related variants differing in pulmonary metastatic potential. Clin. Exp. Metastasis 18: 261-271, 2000. PubMed: 11315100 89454: Khanna C, et al. Metastasis-associated differences in gene expression in a murine model of osteosarcoma. Cancer Res. 61: 3750-3759, 2001. PubMed: 11325848 89468: Khanna C, et al. The membrane-cytoskeleton linker ezrin is necessary for osteosarcoma metastasis. Nat. Med. 10: 182-186, 2004. PubMed: 14704791 |
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K7M2-pCI Neo
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