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- 生长状态：
贴壁生长
- ATCC Number：
CRL-2349™
- 运输方式：
冻存运输
- 器官来源：
肺
- 物种来源：
仓鼠
- 是否是肿瘤细胞：
0
- 细胞类型：
成纤维细胞
- 库存：
大量
- 细胞形态：
成纤维样
| Designations: | XR-V15B | ||
| Depositors: | G Chu | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Cricetulus griseus | ||
| Morphology: | fibroblast |
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| Source: | Organ: lung Cell Type: fibroblast |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | XR-V15B is a X-ray sensitive Chinese Hamster Lung fibroblast cell line isolated from an ethylnitrosourea (ENU)-mutagenized cell population of V79 cells on the basis of its hypersensitivity to X-rays. These cells belong to X-ray complementation group 5 and are mutant in the p86 subunit of the Ku autoantigen. Ku has been shown to be involved in DNA double-strand break repair and V(D)J recombination. Cells will revert to wild type on treatment with azacytidine. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:8 to 1:12 is recommended Medium Renewal: Every 2 to 3 days Remove medium, rinse quickly with 0.25% trypsin, 0.03% EDTA solution, add 1-2 ml additional trypsin solution and allow flasks to set at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new flasks. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| References: | 37304: Rathmell WK, Chu G. Involvement of the Ku autoantigen in the cellular response to DNA double-strand breaks. Proc. Natl. Acad. Sci. USA 91: 7623-7627, 1994. PubMed: 8052631 37306: Errami A, et al. Ku86 defines the genetic defect and restores X-ray resistance and V(D)J recombination to complementation group 5 hamster cell mutants. Mol. Cell. Biol. 16: 1519-1526, 1996. PubMed: 8657125 37309: Smider V, et al. Restoration of X-ray resistance and V(D)J recombination in mutant cells by Ku cDNA. Science 266: 288-291, 1994. PubMed: 7939667 37320: Zdzienicka MZ, et al. Characterization of an X-ray-hypersensitive mutant of V79 Chinese hamster cells. Mutat. Res. 194: 239-249, 1988. PubMed: 2460764 |
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文献和实验Optimizing Particle-Mediated Epidermal Delivery of an Influenza DNA Vaccine in Ferrets
to the model antigens influenza virus hemagglutinin and hepatitis B core antigen. Results from these studies support the use of the PowderJect XR1 device in ferrets for the study of prophylactic and therapeutic DNA vaccines against clinically important diseases
应用案例 | 抑郁症患者的福音，Cell 杂志发文揭示心理韧性的环路机制
于 A1 的 MG 神经元突触前的酪氨酸激酶 B（tropomyosin receptor kinase B, TrkB）受体，从而激活投射到 A1 的 MG 神经元，促进接受 MG 投射的 PV 神经元兴奋（图 1）。 图 1 丘脑-初级听觉皮层环路介导慢性压力模型中「心理韧性」的产生综合大量神经环路和操控手段，研究人员进一步发现光遗传学模拟 MGA1 神经元的短期超极化，而不仅仅是激活 MGA1 神经元，在多种动物模型中引发了应对应激的内源性心理韧性机制，在多种动物模型中实现快速长效的抗抑郁样
问：我看很多文献中涉及细胞离心时一般都是用多少克力来表示。请问这个单位和离心机的转速如何换算呢？应该与离心机的半径有关吗？特此请教大侠，谢谢！答；RCF（相对离心力）=0.00001118XRXN（2）（2为平方）；R为离心管底端到轴心距离；N为转速例：离心半径12CM，转速2000R/MIN，RCF=0.00001118X12X2000（2）=536.64g原理：F=W（2）XR---------（A） W=2X3.1415926XN/60----------（B） g=1.1×10
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