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- 2025年11月21日
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- 技术资料
- 器官来源：
胚胎
- 品系：
NIH/Swiss
- 细胞形态：
成纤维样
- 库存：
大量
- 运输方式：
冻存运输
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- ATCC Number：
CRL-2202™
- 生长状态：
贴壁生长
- 年限：
embryo
| Designations: | PA317 LXSN | ||
| Depositors: | DA Galloway | ||
| Biosafety Level: | 2 [Cells contain human papilloma viral DNA sequences ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus | ||
| Morphology: | fibroblast |
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| Source: | Organ: embryo Strain: NIH/Swiss |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | Note: The user of the PA317 LXSN cell line agrees to indemnify and hold harmless the United States, the ATCC , Denise A. Galloway and Fred Hutchinson Cancer Research Center, Seattle, Washington from any claims, costs, damages, or expenses resulting from any injury (including death), damage or loss that may arise from the use of the cell line either directly (including use for diagnostic purposes) or in the preparation of a product. The user assumes all risks and responsibilities in connection with the receipt, handling, storage and use of the material. | ||
| Age: | embryo | ||
| Comments: | PA317 LXSN is a packaging cell line developed by transfection of the retrovirus vector pLXSN into the Psi-2 ecotropic packaging cell line. Virions produced from the transfected Psi-2 cells were used to infect the amphotropic packaging line PA317, and infected cells were selected in medium containing G418. PA317 LXSN cells produce an amphoteric retrovirus with an empty neo control vector, 5' long terminal repeat (LTR) from the Moloney murine leukemia virus (MoMuLV) multiple cloning region and 3' LTR from MoMuLV. The vector also contains a gene controlling resistance to neomycin transcribed from the SV40 promoter. This line is useful as a negative control for the use of PA317 LXSN 16E6E7 (see ATCC CRL-2203 ). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:12 is recommended Medium Renewal: Every 2 to 3 days Remove medium, rinse flask with fresh 0.25% trypsin, 0.02% EDTA and remove trypsin, Add an additional 1 to 2 ml of trypsin solution, and allow the flask to sit at room temperature (or 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks. |
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| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| References: | 22566: Halbert CL, et al. The E7 gene of human papillomavirus type 16 is sufficient for immortalization of human epithelial cells. J. Virol. 65: 473-478, 1991. PubMed: 1845902 | ||
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文献和实验干货 | CUT and Tag 核心酶 Hyperactive PA/PG-Tn5 Transposase 大解密
比高、实验周期短（ 1 天实现从细胞到文库构建）、可重复性好等显著优势，尤其适用于早期胚胎发育、干细胞、肿瘤以及表观遗传学等研究领域。 图 1. CUT&Tag 原理图 由于 CUT&Tag 主要是针对极低细胞起始量进行实验，因此对核心酶原料有着极高的要求。CUT&Tag 技术的核心原料酶是 Hyperactive PA/PG-Tn5 Transposase ，它具有高活性，对微量 DNA 有高灵敏度和高亲和力，能有效抓取数十个细胞中的有限结合位点等特点
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