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- 细胞形态：
成纤维样
- 库存：
大量
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 运输方式：
冻存运输
- 年限：
embryo
- 器官来源：
胚胎
- 生长状态：
贴壁生长
- ATCC Number：
CCL-226™
- 品系：
C3H
| Designations: | C3H/10T1/2, Clone 8 | ||
| Depositors: | C Heidelberger | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus | ||
| Morphology: | fibroblast |
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| Source: | Organ: embryo Strain: C3H |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host | ||
| Tumorigenic: | No | ||
| Antigen Expression: | H-2k | ||
| Cytogenetic Analysis: | Mouse karyotype with a modal number of 80 chromosomes. | ||
| Age: | embryo | ||
| Comments: | C3H/10T1/2, Clone 8 was isolated by C. Reznikoff, D. Brankow and C. Heidelberger in 1972 from a line of C3H mouse embryo cells. The cells are very sensitive to post confluence inhibition of cell division, do not produce tumors in syngeneic mice, have no background of spontaneous transformation, nor do they contain overt endogenous transforming murine leukemia or sarcoma viruses. The cells are contact sensitive. There is no detectable background spontaneous transformation. They are highly susceptible to transformation by chemical agents. Tested and found negative for ectromelia virus (mousepox). NOTE: THE INOCULATION DENSITY, FEEDING AND HARVESTING SCHEDULES MUST BE FOLLOWED RIGIDLY IF THE LINE IS TO RETAIN ITS ESSENTIAL CHARACTERISTICS. THE BATCH OF SERUM USED FOR GROWTH AND FOR TRANSFORMATION ASSAYS MAY AFFECT BOTH THE MORPHOLOGY OF THIS LINE AND THE RESULTS OBTAINED. Monolayers established and maintained for the standard transformation assay should be free of all foci after 6 weeks. The donor recommends that the line be used between the 5th and 15th passages only. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is Eagle's Basal medium with 2 mM L-glutamine , 1.5 g/L sodium bicarbonate and Earle's BSS. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
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| Subculturing: | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. SUBCULTURE MUST BE DONE BEFORE THE CULTURE REACHES CONFLUENCE. Subcultivation Ratio: Seed new flasks at 2000 viable cells/sq cm. Medium Renewal: Once between subcultures if necessary |
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| Preservation: | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid notrogen vapor temperature |
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| References: | 1208: Reznikoff CA, et al. Quantitative and qualitative studies of chemical transformation of cloned C3H mouse embryo cells sensitive to postconfluence inhibition of cell division. Cancer Res. 33: 3239-3249, 1973. PubMed: 4796800 1209: Terzaghi M, Little JB. Repair of potentially lethal radiation damage in mammalian cells is associated with enhancement of malignant transformation. Nature 253: 548-549, 1975. PubMed: 1167940 1210: Mondal S, Heidelberger C. Transformation of C3H/10T1/2 CL8 mouse embryo fibroblasts by ultraviolet irradiation and a phorbol ester. Nature 260: 710-711, 1976. PubMed: 1264242 22440: Smith GJ, et al. Clonal analysis of the expression of multiple transformation phenotypes and tumorigenicity by morphologically transformed 10T1/2 cells. Cancer Res. 53: 500-508, 1993. PubMed: 8425183 22697: Rapp UR, et al. Endogenous oncornaviruses in chemically induced transformation. I. Transformation independent of virus production. Virology 65: 392-409, 1975. PubMed: 165619 23019: Reznikoff CA, et al. Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division. Cancer Res. 33: 3231-3238, 1973. PubMed: 4357355 33039: Jain MK, et al. Molecular cloning and characterization of SmLIM, a developmentally regulated LIM protein preferentially expressed in aortic smooth muscle cells. J. Biol. Chem. 271: 10194-10199, 1996. PubMed: 8626582 |
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C3H/10T1/2, Clone 8
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