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- 详细信息
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 细胞类型：
T淋巴细胞
- 生长状态：
悬浮生长
- 器官来源：
其他
- 年限：
newborn
- 细胞形态：
淋巴样
- ATCC Number：
CRL-8293™
- 库存：
大量
- 运输方式：
冻存运输
| Designations: | C5/MJ | ||
| Depositors: | President and Fellows of Harvard College | ||
| Biosafety Level: | 2 [Cells Contain RETROVIRUS ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Homo sapiens | ||
| Morphology: | lymphoblast |
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| Source: | Organ: cord blood Cell Type: T lymphocyte; |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: 1983 | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 9,11 D13S317: 12 D16S539: 8,11 D5S818: 13 D7S820: 11,12 THO1: 6,9 TPOX: 8 vWA: 16,17 |
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| Age: | newborn | ||
| Gender: | male | ||
| Comments: | The cells produce Human T cell Leukemia virus (HTLV-I) and HTLV related antigens. The cells express a cell surface glycoprotein of 61000 daltons (45000 daltons when not glycosylated) that is characteristic of HTLV infection. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol: Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at about 2 to 4 X 10(5) cells/ml and maintain between 4 X 10(5) and 1 X 10(6) cells/ml. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Twice per week |
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| Preservation: | Freeze medium: complete growth medium, 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005 recommended serum:ATCC 30-2020 Cell culture tested DMSO:ATCC 4-X |
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| References: | 4002: Essex ME, Lee TH. Method and products for detection of human T cell leukemia virus. US Patent 4,743,678 dated May 10 1988 22945: Popovic M, et al. Isolation and transmission of human retrovirus (human t-cell leukemia virus). Science 219: 856-859, 1983. PubMed: 6600519 |
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C5/MJ
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