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- 询价记录
- 文献和实验
- 技术资料
- 器官来源：
肾脏
- 运输方式：
冻存运输
- 物种来源：
牛
- 是否是肿瘤细胞：
0
- 年限：
adult
- 相关疾病：
正常
- ATCC Number：
CCL-22™
- 细胞形态：
上皮样
- 库存：
大量
- 生长状态：
贴壁生长
| Designations: | MDBK (NBL-1) | ||
| Depositors: | S Madin, NB Darby | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Bos taurus | ||
| Morphology: | epithelial |
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| Source: | Organ: kidney Disease: normal |
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| Cellular Products: | keratin | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | detection and quantitation of replication competent retroviruses [92434 ] transfection host |
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| Virus Resistance: | poliovirus 2 | ||
| Cytogenetic Analysis: | Chromosome Frequency Distribution 50 Cells: 2n = 60. The stemline chromosome number is hypodiploid with 2S component occurring at 5%., There were a total of 11-14 marker chromosomes (4-5 metacentric, 3-4 submetacentric and 4-5 acro-telocentric) common to most hypodiploid metaphases. The X was monosomic. Neither HSR chromosomes nor DM's were seen. | ||
| Age: | adult | ||
| Gender: | male | ||
| Comments: | The MDBK cell line was derived from a kidney of an apparently normal adult steer, February 18, 1957, by S.H. Madin and N.B. Darby. It was ascertained in 1973 that the MDBK line was infected with bovine viral diarrhea virus (BVDV). In 1982 R.A. Van Deusen of the NVSL, USDA, Ames, Iowa informed the ATCC that they had found an MDBK free of BVDV infection. The cells were originally obtained in July, 1967 at passage 96 from the ATCC as a frozen ampule (F-145). Extensive testing (35 passages) by the NVSL found no evidence of any viral contamination in this particular progeny of MDBK. The cell line was submitted to the American Type Culture Collection by R.A. Van Deusen in passage 110 in August, 1982. The cells are positive for keratin by immunofluorescence. The line has been shown to be free of bovine viral diarrhea virus (BVDV). [22883 ] |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Twice per week Rinse the cell sheet twice with fresh 0.25% trypsin, 0.03% EDTA solution, and remove the trypsin solution. Allow the flask to stand at room temperature for 10 to 15 minutes, add fresh medium, aspirate and dispense into new flasks. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 | ||
| References: | 22217: Madin SH, Darby NB Jr.. Established kidney cell lines of normal adult bovine and ovine origin. Proc. Soc. Exp. Biol. Med. 98: 574-576, 1958. PubMed: 13567776 22883: Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438 28301: Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321 32728: Russell DW, Miller AD. Foamy virus vectors. J. Virol. 70: 217-222, 1996. PubMed: 8523528 92434: USEPA Manual of Methods for Virology - EPA publication. Washington, DC:Environmental Protection Agency;EPA EPA 600/4-84/013 (R9), 2001 |
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文献和实验Specific Cell Cycle Synchronization with Butyrate and Cell Cycle Analysis
Synchronized cells have been invaluable in many kinds of cell cycle and cell proliferation studies. Butyrate induces cell cycle arrest and apoptosis in Madin Darby Bovine Kidney (MDBK) cells. We explore the possibility of using butyrate
肺腺癌细胞 400元 MCF7 [MCF-7] TCHu 74 人 乳腺癌细胞 800元 MDA-MB-231 TCHu104 人 乳腺癌细胞 800元 MDA-MB-435S TCHu 36 人 乳腺导管癌 1000元 MDA-MB-453 TCHu 35 人 乳腺癌细胞 400元 MDA-MB-468 TCHu120 人 乳腺癌细胞 1000元 MDBK (NBL-1) GNO 7 牛 牛肾细胞 400元 MDCK (NBL
MDBK (NBL-1) 牛肾细胞 Mv.1.Lu(NBL-7) 貂肺上皮细胞 MDCK (NBL-2) 狗肾细胞 EBTr 牛胚气管细胞 F81 猫肾细胞 PIEC 猪髋动脉内皮细胞 CRFK 猫肾细胞 ＊FRhK-4 恒河猴胚肾细胞 ＊VERO C1008 (E6) 非洲绿猴肾细胞 Pt K1 (NBL-3) 长鼻袋鼠
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