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- 库存：
大量
- 器官来源：
大脑
- 组织来源：
cerebellum
- 品系：
C57BL/6
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 细胞类型：
其他细胞类型
- 年限：
8 days juvenile
- 运输方式：
冻存运输
- ATCC Number：
CRL-2540™
- 生长状态：
贴壁生长
- 细胞形态：
神经元
| Designations: | C8-B4 | ||
| Depositors: | B Pessac, D Trisler | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus | ||
| Morphology: | neuronal |
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| Source: | Organ: brain Strain: C57BL/6 Tissue: cerebellum Cell Type: monocyte/macrophage microglia; spontaneously transformed |
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| Cellular Products: | glutamate [49701 ] | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Antigen Expression: | CD4 [49701 ] | ||
| Age: | 8 days juvenile | ||
| Comments: | Clonal permanent cell lines with astrocytic or microglial properties have been established from explant cultures of 8-day postnatal mouse cerebella after in vitro spontaneous transformation. [48963 ] [49701 ] Sister flasks of lethally irradiated astrocytes, which are known to synthesize the macrophage-microglia growth factor, M-CSF, were used as a substrate for cloning the cells. [48963 ] [49701 ] The cell lines were derived in a multistage process. Slowly proliferating foci with several morphologies appeared 4 months after initiation of the cultures and became progressively enriched by cells with a homogeneous appearance. [48963 ] The astrocyte type I cloned cell line named C8-D1A is available as ATCC CRL-2541 , the astrocyte type II cloned cell line named C8-S is available as ATCC CRL-2535 and the astrocyte type III cloned cell line named C8-D30 is available as ATCC CRL-2534. The C8-B4 clone expresses classical microglial markers (MAC1, F4/80, 2-4G2) and appears to be derived from a committed microglial precursor since it does not express differentiation antigens present during the early stage of the monocytic lineage. [49701 ] C8-B4 cells synthesize the CD4 molecule and produce and release large amounts of glutamate. [49701 ] |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
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| Subculturing: | Protocol: NOTE: The cells do not become confluent.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Doubling Time: | 48 to 72 hrs | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 purified RNA:ATCC CRL-2540R 0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101 Cell culture tested DMSO:ATCC 4-X |
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| References: | 48963: Alliot F, Pessac B. Astrocytic cell clones derived from established cultures of 8-day postnatal mouse cerebella. Brain Res. 306: 283-291, 1984. PubMed: 6466977 49701: Alliot F, et al. A spontaneously immortalized mouse microglial cell line expressing CD4. Brain Res. Dev. Brain Res. 95: 140-143, 1996. PubMed: 8873987 |
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文献和实验相关实验
网络 七、C8分子 C8是由α、β、γ三条肽链组成的三聚体糖蛋白，分子量为155kDa。其中α链和β链均为64kDa,γ链为22kDa。α链和γ链间以二硫键共价结合，而α链与β链间则为非共价键结合（图5-10）。C8分子中也含有TSP-1和LDL受体结构功能域。在C8α和C8β多肽链的中央（157-501个氨基酸残基间），几科
化，其他已知细胞松弛素 B还可抑制糖的输送，可以考虑细胞松弛素 B与细胞膜间的相互作用。此外还分离出数种具有类似结构和功能的物质，分别命名为细胞松弛素 A、 C、 D、 E、 F。
细胞色素b563 cytochrome b563 亦称细胞色素 b6 。存在于高等植物至藻类的叶绿体中，与类囊体膜结合紧密。含有原正铁血红素，氧化还原 （β带）和 434nm（γ带或 So－ ret带）。为发动光合磷酸化的环状电子传递系统的一个成分，可被光化系统Ⅰ所还原和氧化。
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C8-B4
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