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- 运输方式：
冻存运输
- ATCC Number：
HB-8110™
- 库存：
大量
- 生长状态：
悬浮生长
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 免疫类型：
IgG1
- 细胞形态：
淋巴样
| Designations: | B38.1 | ||
| Depositors: | NIH | ||
| Isotype: | IgG1 | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against human mammary tumor cells | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with membrane enriched fractions of extracts from either of two human breast tumor metastases to the liver (Met 1 and Met 2). Spleen cells were fused with NS-1 myeloma cells. The antibody reacts weakly with extracts of both metastases and with live cells, and strongly with live BT-20 (ATCC HTB-19 ), MCF7 (ATCC HTB-22 ) and or ZR-75-1 (ATCC CRL-1500 ) mammary carcinoma cells. The antibody does not react with liver extracts or a variety of live normal and sarcoma cells. |
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| Propagation: | ATCC complete growth medium: Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20% |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:5 is recommended Medium Renewal: 3 times per week Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Preservation: | culture medium, 92.5%; DMSO, 7.5% | ||
| References: | 1177: Colcher D, et al. A spectrum of monoclonal antibodies reactive with human mammary tumor cells. Proc. Natl. Acad. Sci. USA 78: 3199-3203, 1981. PubMed: 6789331 3537: Colcher D, et al. Process for producing monoclonal antibodies reactive with human breast cancer. US Patent 4,522,918 dated Jun 11 1985 |
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文献和实验【求助】测试p38功能变化仅测磷酸化的P38就可以了，为何还要测总P38呢？
伽利略 我一直都不太明白，请达人解释下，谢谢 bigbang_0_0 蛋白质功能上的变化包括质变和量变两种，所以要检测总的p32蛋白水平。 George2 测总P38是为了了解P38的表达背景，以估测激活的P38占总量的比例。 伽利略 我是这样想的，请问有何问题 P38磷酸化水平若增高不论是否P38总量变化，都能说明P38活跃或者激活，不测P38
yzzaici888 大家好，本人最近一直在做p-p38的磷酸化，但是一直都做不好。希望各位大侠帮助解决： 蛋白上样量80-100ug，半干转膜70min，5%BSA封闭70min，一抗CST（#9211） 1：500加，4℃孵育过夜，二抗1:8000加室温70min。曝光出的结果很不好，杂带很多，也分析不出那条是目的条带，且没有趋势，见下图 15001046419
化，其他已知细胞松弛素 B还可抑制糖的输送，可以考虑细胞松弛素 B与细胞膜间的相互作用。此外还分离出数种具有类似结构和功能的物质，分别命名为细胞松弛素 A、 C、 D、 E、 F。
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