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CCD-27Sk

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  • 2025年12月30日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 运输方式

      冻存运输

    • 库存

      大量

    • 相关疾病

      正常

    • ATCC Number

      CRL-1475™

    • 年限

      23 weeks gestation

    • 器官来源

      皮肤

    • 生长状态

      贴壁生长

    • 物种来源

    • 是否是肿瘤细胞

      0

    • 细胞类型

      成纤维细胞

    • 细胞形态

      成纤维样

    Designations: CCD-27Sk
    Biosafety Level: 1
    Shipped: frozen
    Medium & Serum: See Propagation
    Growth Properties: adherent
    Organism: Homo sapiens
    Morphology: fibroblast

    Source: Organ: skin
    Disease: normal
    Cell Type: fibroblast
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    Age: 23 weeks gestation
    Gender: male
    Ethnicity: Black
    Comments: The line was derived from normal skin of a neonate that died of complications following premature birth.
    Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
    Subculturing: Protocol:
    1. Remove and discard culture medium.
    2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
    3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37�C to facilitate dispersal.
    4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
    5. Add appropriate aliquots of the cell suspension to new culture vessels.
    6. Incubate cultures at 37�C.

        Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
        Medium Renewal: 2 to 3 times per week
    Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
    Storage temperature: liquid nitrogen vapor phase
    Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
    recommended serum:ATCC 30-2020

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