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- 详细信息
- 技术资料
- 相关疾病：
其他疾病
- ATCC Number：
CRL-1351™
- 年限：
20 years
- 器官来源：
皮肤
- 运输方式：
冻存运输
- 生长状态：
贴壁生长
- 细胞形态：
成纤维样
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 库存：
大量
| Designations: | Ce Wal | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | fibroblast |
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| Source: | Organ: skin Disease: poikiloderma |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Age: | 20 years | ||
| Gender: | male | ||
| Comments: | The tissue donor suffered from severe poikiloderma, without tumors. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: 2 to 3 times per week Remove spent medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit at 37C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 8 to 10 days. |
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Ce Wal
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