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- 详细信息
- 技术资料
- 库存：
大量
- 相关疾病：
其他疾病
- ATCC Number：
TIB-190™
- 年限：
adult
- 免疫类型：
IgG
- 细胞形态：
淋巴样
- 生长状态：
悬浮生长
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 运输方式：
冻存运输
- 细胞类型：
淋巴母细胞
| Designations: | CESS | ||
| Depositors: | P Ralph | ||
| Isotype: | IgG | ||
| Biosafety Level: | 2 [Cells Contain HERPESVIRUS ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Homo sapiens | ||
| Morphology: | lymphoblast |
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| Source: | Disease: myelomonocytic leukemia Cell Type: lymphoblast |
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| Cellular Products: | immunoglobulin; surface immunoglobulin (sIg+); Epstein-Barr virus (EBV) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | CESS cells can be used to assay T-cell replacing factor (TRF). | ||
| Receptors: | complement | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 10,11 D13S317: 12 D16S539: 12 D5S818: 11,12 D7S820: 10,12 THO1: 7,9.3 TPOX: 8,9 vWA: 16,17 |
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| Age: | adult | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | Isolated from the peripheral blood of an adult Caucasian male patient with myelomonocytic leukemia after incubation with Epstein-Barr Virus. The cells are EBNA +. CESS cells can be used to assay T-cell replacing factor (TRF). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(5) viable cells/ml. Interval: Maintain cultures at a cell concentration between 2 X 10(5) and 1 X 10(6) cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
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| References: | 1145: Muraguchi A, et al. T cell-replacing factor-(TRF) induced IgG secretion in a human B blastoid cell line and demonstration of acceptors for TRF. J. Immunol. 127: 412-416, 1981. PubMed: 6972960 1235: Miki Y, et al. The requirement for esterase activation in T cell replacing factor (TRF)-induced IgG production in a human B blastoid cell line. J. Immunol. 128: 675-678, 1982. PubMed: 6798119 1236: Kishimoto T, et al. IgG induction in a human B cell line by red cell-mediated microinjection of the cytoplasm from T cell factor-stimulated B cells. J. Immunol. 129: 1367-1371, 1982. PubMed: 6980930 1237: Miki Y, et al. Induction of IgG production in a human monoclonal B lymphoblastoid cell line by a B cell-specific monoclonal antibody. J. Immunol. 129: 1921-1925, 1982. PubMed: 6981668 22879: Bradley TR, et al. Cell lines derived from a human myelomonocytic leukaemia. Br. J. Haematol. 51: 595-604, 1982. PubMed: 6980663 |
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