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- 详细信息
- 技术资料
- 器官来源：
胸腺
- 库存：
大量
- 年限：
newborn
- 物种来源：
狗
- 是否是肿瘤细胞：
0
- 相关疾病：
正常
- ATCC Number：
CRL-1430™
- 运输方式：
冻存运输
- 生长状态：
贴壁生长
| Designations: | Cf2Th | ||
| Depositors: | WA Nelson-Rees | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Canis familiaris | ||
| Morphology: | |
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| Source: | Organ: thymus Disease: normal |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host | ||
| Age: | newborn | ||
| Gender: | female | ||
| Comments: | The line has been used to propagate retroviruses from cat embryo and baboon lung and kidney. The cells are also susceptible to xenotropic retroviruses from AKR and BALB/c mice. |
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| Propagation: | ATCC complete growth medium: Dulbecco's modified Eagle's medium with non-essential amino acids, 80%; fetal bovine serum, 20% |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: 2 to 3 times per week Remove medium, add fresh 0.25% trypsin, 0.03% EDTA for 2 to 3 minutes, remove trypsin and let the culture sit at 37C for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. |
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| References: | 22649: Nelson-Rees WA, et al. Source, alterations, characteristics and use of a new dog cell line (Cf2Th). In Vitro 12: 665-669, 1976. PubMed: 190163 32500: Campbell M, et al. The simian foamy virus type 1 transcriptional transactivator (Tas) binds and activates an enhancer element in the gag gene. J. Virol. 70: 6847-6855, 1996. PubMed: 8794326 32728: Russell DW, Miller AD. Foamy virus vectors. J. Virol. 70: 217-222, 1996. PubMed: 8523528 |
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Cf2Th
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