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- 详细信息
- 技术资料
- 器官来源：
脾
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 库存：
大量
- 品系：
C57BL/6-TL+
- 运输方式：
冻存运输
- ATCC Number：
CRL-8179™
- 细胞形态：
淋巴样
- 生长状态：
悬浮生长
| Designations: | Cl. Ly1+2-/9 | ||
| Depositors: | Dana Farber Cancer Inst. | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus | ||
| Morphology: | lymphoblast |
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| Source: | Organ: spleen Strain: C57BL/6-TL+ |
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| Cellular Products: | interleukin-3 (interleukin 3, IL-3); T cell growth factor 2 (TCGF2); mast cell growth factor 2 (MCGF2); B cell stimulating factor 1 (BSF-1) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | This is a cloned T cell line derived from C57BL/6TL+ spleen cells. The cells produce IL-3, TCGF2, MCGF2 and BSF-1. |
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| Propagation: | ATCC complete growth medium: RPMI 1640 with 2.0 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 80%; fetal bovine serum, 10%; rat T-STIM with Con A, 10%.Rat T-STIM with Con A can be obtained from Becton Dickinson, Catalogue No. 354115 Temperature: 37.0°C |
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| Subculturing: | Medium Renewal: Every 2 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 0.5 X 10 exp5 and 3 X 10 exp6 cells/ml. |
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| Preservation: | RPMI 1640 medium 75%; fetal bovine serum, 20%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
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| References: | 21910: Cantor HI, Nabel G. Lymphocyte growth factor. US Patent 4,613,459 dated Sep 23 1986 22305: Nabel G, et al. Use of cloned populations of mouse lymphocytes to analyze cellular differentiation. Cell 23: 19-28, 1981. PubMed: 6971154 23272: Nabel G, et al. Multiple biologic activities of a cloned inducer T-cell population. Proc. Natl. Acad. Sci. USA 78: 1157-1161, 1981. PubMed: 6785749 23454: Mosmann TR, et al. Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. J. Immunol. 136: 2348-2357, 1986. PubMed: 2419430 |
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Cl. Ly1+2-/9
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