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- 详细信息
- 文献和实验
- 技术资料
- 库存:
225
- 英文名:
Recombinant Human Tumor Necrosis Factor-alpha
- 保质期:
长期
- 供应商:
北京百奥莱博科技有限公司
- 保存条件:
冷冻(-20℃)
- 规格:
10μg
特别提示:包括重组人TNF-α在内,本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!
产品名称:重组人TNF-α
英文名称:Recombinant Human Tumor Necrosis Factor-alpha
产品货号:GS4376
产品规格:10μg
分子量:Approximately 17.5kDa,a single non-glycosylated polypeptide chain containing 158 amino acids.
储存条件:冷冻(-20℃)
概述
Tumor necrosis factor alpha(TNF-α),also called cachectin,is the best-know member of the TNF-family,which can cause cell death.This protein is produced by neutrophils,activated lymphocytes,macrophages,NK cells,LAK cells,astrocytes endothelial cells,smooth muscle cells and some transformed cells.TNF-α occurs as a secreted,soluble form and as a membrane-anchored form,both of which are biologically active.The naturally-occurring form of TNF-α is glycosylated,but non-glycosylated recombinant TNF-α has comparable biological activity.The biologically active native form of TNF-α is reportedly a trimer.Human and murine TNF-α show approximately 79%homology at the amino acid level and cross-reactivity between the two species.Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
| 外观 | Sterile Filtered White lyophilized(freeze-dried)powder. |
| 纯度 | >98%by SDS-PAGE and HPLC analyses. |
| 来源 | Escherichia coli. |
| 生物活性 | Fully biologically active when compared to standard.The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05ng/ml,corresponding to a specific activity of>2.0×107 IU/mg in the presence of actinomycin D. |
| 成分 | Lyophilized from a 0.2μmfiltered concentrated solution in 20mMPB,10 mM Nacl,pH 7.0. |
| 内毒素 | Less than 1.0 EU/μg of rHuTNF-α as determined by LAL method. |
| 复溶 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20?C.Further dilutions should be made in appropriate buffered solutions. |
| 氨基酸序列 | MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL |
我公司销售的重组人TNF-α价格,Recombinant Human Tumor Necrosis Factor-alpha,重组人Tumor Necrosis Factor-alpha,TNF-α, Human,重组Tumor Necrosis Factor-alpha质量上佳,价格普惠,欢迎广大新老客户踊跃订购。
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