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Ready-to-Assay 5-HT1A Frozen Cells
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文献和实验Thymidine-Incorporation Assay for Rat-1a cells
Thymidine-Incorporation Assay for Rat-1a cells Overview This method of Peter Coward, Ph.D. in the Conklin Lab was used in Coward, et al (1998) Controlling signaling with a specifically designed Gi-coupled receptor Proc. Natl
Frozen Permanents YEAST 1) grow culture to stationary in YEPD 2) Add 0.8ml of culture to 0.8ml of 30% glycerol in labelled freezer vials 3) vortex and place at -80o C 4) for strains with plasmids, pick
Apoptosis TUNEL Assay (frozen sections)
Protocol for Frozen Sections: Warm 150ml 4% Paraformaldehyde/1x PBS to RT. Fix slides in it, 20 min., RT. 1x PBS rinse, 2 times. 1x PBS, 30 min., RT. Begin chilling Triton/SSC on ice. 0.1% Triton/ 0.1% Sodium
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