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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8℃
- 英文名:
无
- 库存:
999
- 供应商:
上海源叶生物科技有限公司
- CAS号:
3438-55-9
- 规格:
1g/5g
| 规格: | 1g | 产品价格: | ¥75.0 |
|---|---|---|---|
| 规格: | 5g | 产品价格: | ¥340.0 |
3438-55-9 / 3438-55-9 其他生化试剂(3438-55-9);【名称】:5-Bromo-4-chloro-6-methylpyrimidine 【英文名】: 【CAS】:3438-55-9 【货号】:S98824 【规格】:97% 【分子式】:C5H4BrClN2 【分子量】:207.46 【熔点】:\N 【用途】:\N
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文献和实验DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONS
) through the oil to sample. (It's 0.3 ul per 15ul original volume, so adjust as necessary). Incubate overnight at 55 C. Day 3: (Repeat:) Add 0.3 ul fresh conc Proteinase K (20 mg/ml stock) through the oil to sample. (It's 0.3 ul per 15 ul original
Single Primer ("Semi-Random") P
to about 1 ng/µL 3 µL "T/TS" @ 0.4 u Taq/µL -------- 30 µL Cycle as follows: 30"x94°C 20 cycles 0"x94°C 0"x55°C 1'x72° S=9 30 cycles 0"x94°C 0"x40°C 1'x72° S=6 30 cycles 0"x94°C 0"x55°C 1'x72° S=9 Clean up: Add 1 µL ExoI
that binds to methylated DNA [J] Cell, 1992, 69: 905-914.[55]Yegnasubramanian S, Lin X, Haffner M C, et al. Combination of methylated-DNA precipitation and methylation-sensitive restriction enzymes (COMPARE-MS) for the rapid, sensitive and quantitative
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