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- 详细信息
- 文献和实验
- 技术资料
- 库存:
99
- 供应商:
爱必信(上海)生物科技有限公司
- 规格:
500mg
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文献和实验DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONS
) through the oil to sample. (It's 0.3 ul per 15ul original volume, so adjust as necessary). Incubate overnight at 55 C. Day 3: (Repeat:) Add 0.3 ul fresh conc Proteinase K (20 mg/ml stock) through the oil to sample. (It's 0.3 ul per 15 ul original
Single Primer ("Semi-Random") P
to about 1 ng/µL 3 µL "T/TS" @ 0.4 u Taq/µL -------- 30 µL Cycle as follows: 30"x94°C 20 cycles 0"x94°C 0"x55°C 1'x72° S=9 30 cycles 0"x94°C 0"x40°C 1'x72° S=6 30 cycles 0"x94°C 0"x55°C 1'x72° S=9 Clean up: Add 1 µL ExoI
that binds to methylated DNA [J] Cell, 1992, 69: 905-914.[55]Yegnasubramanian S, Lin X, Haffner M C, et al. Combination of methylated-DNA precipitation and methylation-sensitive restriction enzymes (COMPARE-MS) for the rapid, sensitive and quantitative
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