Biotin-BMCC is a sulfhydryl-reactive biotinylation reagent with a long spacer arm.Maleimide-activated reagents are
effective for protein modification of sulfhydryl groups.Maleimide groups react efficiently and specifically with free(reduced)
sulfhydryls at pH 6.5-7.5 to form stable thioether bonds(Figure 1).Most proteins have cysteine residues whoseside-chain
sulfur atoms typically occur in pairs as disulfide bonds.Reduction of these disulfide bonds exposes the sulfhydryl group
required as a target for biotinylation with maleimide-activated reagents.Alternatively,sulfhydryl groups can be addedto
molecules using various modification reagents(see subsequent Important Product Information Section).
应用
Biotin-BMCC is not directly soluble in water or aqueous buffers;however,if it is first dissolved in dimethylsulfoxide(DMSO)or other organic
solvent,then it can be diluted and remain soluble in an aqueous buffer at typical reaction concentrations.
组份
50mg/bottle
技术规格
分子量
533.68
间隔臂
32.6?
纯度
>95%on TCL
反应pH值
acidic or neutral pH
溶解性
~4.5 mg/ml(8.5mM)in DMSO(slightly less soluble in DMF)