Bse118I内切酶北京厂家

特别提示：包括Bse118I内切酶在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：Bse118I内切酶
英文名称：Bse118I
产品货号：GS2081
产品规格：100U

储存条件：冷冻（-20℃）

技术规格
 

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 65℃ in a total reaction volume of 50μl.
Recognition sites	R↑CCGGY
来源	Bacillus stearothermophilus 118
Assayed on	Lambda DNA
Optimal buffer	O(50mMTris-HCl(pH 7.6 at 25℃);10 mM MgCl2;100 mM NaCl;1 mM DTT.)
Optimal temperature	65℃
Storage conditions	10 mM KH2PO4(pH 7.5);100 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;100 μg/ml BSA;50%glycerol;Store at -20℃.
Ligations	After 2-fold overdigestion with enzyme 95%of the DNA fragments can be ligated and recut.
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 2 u.a.of enzyme for 16hoursat 65℃.
Methlation effect	Methylation sensitivity not tested
Thermal inactivation	enzyme is inactivated by incubation at 80℃ for 20 mins.
Campatible Ends	BsrFI,BssAI,Cfr10I

我公司销售的Bse118I内切酶北京厂家，质量上佳，价格普惠，欢迎广大新老客户踊跃订购。