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文献和实验Purification of DNA‐Binding Proteins Using Biotin/Streptavidin Affinity Systems
Streptavidin (Celltech Ltd.; may be stored as 5 mg/ml stock for at least 2 months) DEAE membrane (Schleicher & Schuell NA45
on the beads was detected using biotinylated antigen/streptavidin-phycoerythrin. The method was demonstrated by isotyping antibodies directed against an anti-CD11a antibody therapeutic (RAPTIVA� /efalizumab) from the serum of a cynomolgus monkey hyper-immunized
4F in the working solution. Measurement of avidin in a crude sample is exemplified by mixing 8 pmol of B4F with various amounts of diluted egg white in a volume of 1 mL. Hereby, the extent of fluorescence quenching linearly correlates
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