Akt Antibody+Phospho-Akt(Ser47

Application: WB Species:rat; Sample:rat

Fig. 7. Effect of quercetin on TrkA/AKT pathway signaling in PASMCs. (A) The expression levels of p-TrkA/TrkA and p-AKT/AKT were measured by Western blotting in PASMCs in hypoxia and treated with increasing concentrations of quercetin for 24 h. (B) Results were quantified by densitometry analysis of the bands form (A) and then normalization to GAPDH protein. (C) Effects of NGF, GW441756, and quercetin on the expression of p-TrkA/TrkA and p-AKT/AKT in PASMCs in hypoxia. (D) Densitometric quantification of the bands were shown. (E) The effect of quercetin on PASMCs migration ability under the hypoxia condition treated with or without NGF (50 μg/L). (F) Quantification of the number of cells migrating through the polycarbonate membrane of average of 3 independent experiments. (G) Quantification of the Flow cytometry analysis results to confirm the effects of quercetin on PASMCs apoptosis and (H) cell cycle under the hypoxia condition treated with NGF (50 ug/L). *Po0.05, **Po0.01 compared with control; #Po0.05, ##Po0.01 compared with hypoxia and quercetin treated groups; &Po0.05, &&Po0.01 compared with NGF treated PASMCs.

Application: WB Species:human; Sample:Not available

Fig. 4 Fucoxanthin promotes apoptosis via inhibition of PI3K/Akt/ mTOR signaling pathway in glioblastoma cells. a Cell lysates were electrophoresed and Akt, p-Akt, mTOR and p-mTOR proteins were detected by their respective specific antibodies in indicated concentrations

Application: WB Species:human; Sample:Not available

Treatment with angelicin alters the protein expression levels of PI3K, p-Akt and total Akt in HepG2 and Huh-7 cells in vitro. (A) Representative blots demonstrating PI3K, p-Akt and total Akt protein expression levels in HepG2 and Huh-7 cells, following treatment with various concentrations of angelicin. (B) PI3K blots were semi-quantified using densitometry analysis of the protein bands and normalized to GAPDH. (C) p-Akt/Akt blots were semi-quantified using densitometry analysis of the protein bands. (D) Effects of LY294002 on angelicin-induced apoptosis. Cells were treated with the PI3K inhibitor LY294002 (3 mM) for 1 h prior to treatment with angelicin. The percentage of apoptotic cells following treatment with angelicin in the presence or absence of LY294002 was assessed using Annexin V-fluorescein isothiocyanate/propidium iodide staining and flow cytometry. Data are expressed as the mean ± standard deviation of 3 independent experiments.

Application: WB Species:human; Sample:Not available

Figure 5: Effects of RLX on the expression of pAkt and apoptosis proteins

Application: WB Species:human; Sample:Not available

Fig 5. The expression of p-p65, p-AKT and Bcl-2 (protein level) in U2-OS cells treated by LY294002 or/and Estrogen receptor β (ERβ) siRNA in the presence of 10-10 M E2.

Application: WB Species:human; Sample:Not available

(b) Western bolt shown that inhibited MEK, both decrease MNK2 expression and eIF4E phosphorylation. Inhibited AKT, decreased MNK2 and eIF4E, 4EBP1 phosphorylation.

Application: WB Species:mouse; Sample:mouse

Fig. 9. Chrysin inhibits the phosphorylation of Akt and ERK1/2. The levels of Akt, p-Akt, ERK1/2 and p-ERK1/2 in protein extracts from lung tissues were analyzed with Western blot (A). The bar graph represents percent phosphorylation level compared with the control group (B and C). Densitometric data are expressed as mean ± SEM (#p b 0.05 vs. control group; *p b 0.05 vs. OVA group, n = 6).

Application: WB Species:human; Sample:Not available

Figure 5: BVD-523 synergizes with VS-5584 in AML cells. (A) BxPC-3, MIAPaCa-2, and CFPAC-1 cells were treated with vehicle control or variable concentrations of BVD-523 and VS-5584, alone or in combination, for 48 h. Viable cells were measured by MTT assays. Standard isobologram analyses of the antitumor interactions are shown. The IC50 values of each drug are plotted on the axes; the solid line represents the additive effect, while the points represent the concentrations of each drug resulting in 50% inhibition of proliferation. Points falling below the line indicate synergism whereas those above the line indicate antagonism. (B) HPAC cells were treated with vehicle control, VS, BVD-523 (BVD), or VS plus BVD for 48 h. Cells were fxed with 80% ice-cold ethanol and stained with PI for cell cycle analysis. The percentage of cells with sub-G1 DNA content are graphed as means of triplicates ± SEM from one representative experiment. ***indicates p < 0.001; combined treatment compared to individual treatments and vehicle control. Combination index (CI) values were calculated using CompuSyn software. (C) CI vs. Fa plot (combination index vs. fraction affected) for the cell death data is presented. (D) HPAC cells were treated with vehicle control or the indicated drugs for 48 h. Whole cell lysates were subjected to Western blotting and probed with the indicated antibody. The fold changes for the densitometry measurements, normalized to β-actin and then compared to no drug treatment control, are indicated.