Mouse TH2 Identification Kit contains a PE conjugated antibody to IL-4 and a PerCP conjugated antibody to CD4 as well as optimized cytokine blocking reagents and buffers to aid in identifying TH2 CD4+ T-cell subsets in ex vivo lymphocyte populations or to monitor TH2 CD4+ T-cell differentiation in culture. In addition, TBD has added fixable viability dye that eliminates false positive data resulting from cytokine staining on dead cells that can occur in long term cultures. The TBD Mouse TH2 Identification Kit contains sufficient reagents for 25 3-color tests. Detailed assay instructions are included to assist in analysis and to ensure that the correct cell concentration is obtained during acquisition of sample data. This kit is not designed to be used in the analysis of whole spleen cells in SJL mice.