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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
Invitrogen™
- 规格:
500 µg
nvitrogen™
Human Cot-1 DNA™
Catalog number: 15279011
| Catalog number | Unit size | Price (CNY) | Qty | |
|---|---|---|---|---|
| 15279011also known as 15279-011 | 500 µg | 3,893.40 |
Description
Human Cot-1 DNA® is commonly used to block nonspecific hybridization in microarray screening. It can also be used to suppress repetitive DNA sequences for the direct mapping of human DNA or mapping genomic clones to panels of somatic-cell hybrids for chromosome localization by Southern blotting. Human Cot-1 DNA® is effective as a library-screening probe for somatic-cell hybrid libraries and flow-sorted chromosome libraries made from somatic-cell hybrids.About Cot-1 DNA®
Human Cot-1 DNA® is placental DNA that is predominantly 50 to 300 bp in size and enriched for repetitive DNA sequences such as the Alu and Kpn family members. The amount supplied per package is sufficient for 5–10 Southern or 500 in situ hybridizations.
Performance and quality testing
Purity and DNA size are verified by agarose gel electrophoresis. Concentration is verified spectrophotometrically by diluting Human Cot-1 DNA® 1:100 in 50 mM NaOH and using the conversion factor 0.033 µg/µL/A260. Other methods used to determine concentration may yield varying results.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Reagent Type: | Cot-1 DNA |
|---|---|
| Key Function: | Block Non-Specific Hybridization |
| Product Line: | Cot-1 DNA® |
| Product Size: | 500 µg |
| Shipping Condition: | Approved for shipment on Wet or Dry Ice |
Contents & storage
This DNA is supplied at 1 mg/ml in 10 mM Tris-HCl (pH 7.4), 1 mM EDTA.Store at -20°C.
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