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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
负80度(6个月)
- 保质期:
6个月
- 英文名:
酵母转化配套试剂--PEG/Liac
- 库存:
大量
- 供应商:
上海联迈生物工程有限公司
- 规格:
500ul甘油菌
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文献和实验, resuspend in 2 ml 1 x LiAc/ 0.5 x TE incubate at RT for 10 min combine in tube: 100 µl cells, 10 µl salmon sperm DNA (10 mg/ml), up to 15 µl DNA to be transformed add 700 µl 1 x LiAc / 1 x TE / 40 % PEG mix incubate for 30 min
High Efficiency Yeast Transformation (Gietz Long Protocol)
and remove the LiAc with a micropipette. 11. The basic "transformation mix" consists of: 240 µl PEG (50% w/v) 36 µl 1.0 M. LiAc 50 µl SS-DNA (2.0 mg/ml) X µl Plasmid DNA (0.1 - 10 µg) 34-X µl Sterile ddH2O 360 µl TOTAL Carefully add these ingredients
Yeast Transformation Using Frozen Competent Cells and Single-stranded DNA as a Carrier
2-3 more doublings.) Note: The cells must be grown in glycerol if you are going to freeze them. Glucose-grown cells lose competence upon freezing. 2. Pellet cells gently (~800 x g). Resuspend in 7-8 ml of 1X TE-LiAc . 3. Pellet cells gently. Resuspend in 1 ml
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