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- 库存:
443
- 英文名:
Recombinant Human Fibroblast Growth Factor-9
- 保质期:
长期
- 供应商:
北京百奥莱博科技有限公司
- 保存条件:
冷冻(-20℃)
- 规格:
5μg
特别提示:包括重组人FGF-9在内,本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!
产品名称:重组人FGF-9
英文名称:Recombinant Human Fibroblast Growth Factor-9
产品货号:GS4452
产品规格:5μg
分子量:Approximately 23.3kDa,a single non-glycosylated polypeptide chain containing 207 amino acids.
储存条件:冷冻(-20℃)
概述
Fibroblast growth factor-9(FGF-9)is a member of the fibroblast growth factor(FGF)family.All FGF family members are heparin binding growth factors with a core 120 amino acid(a.a.)FGF domain that allows for a common tertiary structure.FGF-9 plays an important role in the regulation of embryonic development,cell proliferation,cell differentiation and cell migration.This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells.FGF-9 is a monomer and interacts with FGFR1,FGFR2,FGFR3 and FGFR4.The human FGF-9 shares 98%a.a.sequence identity with mouse,rat,equine,porcine,and bovine FGF-9.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
| 外观 | Sterile Filtered White lyophilized(freeze-dried)powder. |
| 纯度 | >95%by SDS-PAGE and HPLC analyses. |
| 来源 | Escherichia coli. |
| 生物活性 | Fully biologically active when compared to standard.The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5ng/ml,corresponding to a specific activity of>2.0×106 IU/mg. |
| 成分 | Lyophilized from a 0.2μmfiltered concentrated solution in PBS,pH 7.4. |
| 内毒素 | Less than 1 EU/μg of rHuFGF-9 as determined by LAL method. |
| 复溶 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions. |
| 氨基酸序列 | APLGEVGNYF GVQDAVPFGN VPVLPVDSPV LLSDHLGQSE AGGLPRGPAV TDLDHLKGIL RRRQLYCRTG FHLEIFPNGT IQGTRKDHSR FGILEFISIA VGLVSIRGVD SGLYLGMNEK GELYGSEKLT QECVFREQFE ENWYNTYSSN LYKHVDTGRR YYVALNKDGT PREGTRTKRH QKFTHFLPRP VDPDKVPELY KDILSQS |
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文献和实验Cell Rep:北京大学李家立课题组揭示阿尔茨海默病易激惹症状发生的神经环路机制
2022 年 11 月 1 日,国际杂志 Cell Reports 在线发表了北京大学麦戈文脑研究中心/北京大学中国药物依赖性研究所研究员李家立课题组研究论文 Hyper-excitability of corticothalamic PT neurons in mPFC promotes irritability in the mouse model of Alzheimer's disease。 该研究揭示了锥体束(pyramidal tract,PT)神经元在阿尔
/ml,不可振荡。 这个步骤即为溶解步骤,非常重要。 1) 一定要用推荐的溶液重悬(或溶解)冻干粉 用于溶解细胞因子或蛋白的溶液千差万别。此例中的重组人IL-4需用水溶解,而重组人IL-2 (产品编号:200-02)则需用100mM Acetic Acid (醋酸)溶解,重组人TGF-beta1 (产品编号:100-21)需用10mMCitric Acid (柠檬酸),pH3.0溶解,重组人FGF-basic(产品编号:100-18B)需用5mMTris,pH7.6溶解
-2 (产品编号:200-02)则需用100mM Acetic Acid (醋酸)溶解,重组人TGF-beta1 (产品编号:100-21)需用10mMCitric Acid (柠檬酸),pH3.0溶解,重组人FGF-basic(产品编号:100-18B)需用5mMTris,pH7.6溶解,重组人FGF-10(产品编号:100-26)需用5mMSodium Phosphate(磷酸钠),pH7.4溶解,重组IL-13(产品编号:200-13)需用20mMMHCl溶解。(注:即使同一重组细胞因子或蛋白,不同
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