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- 详细信息
- 文献和实验
- 技术资料
- 库存:
361
- 英文名:
Recombinant Rat Interleukin-4
- 保质期:
长期
- 供应商:
北京百奥莱博科技有限公司
- 保存条件:
冷冻(-20℃)
- 规格:
5μg
特别提示:包括重组大鼠IL-4在内,本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!
产品名称:重组大鼠IL-4
英文名称:Recombinant Rat Interleukin-4
产品货号:GS4705
产品规格:5μg
分子量:Approximately 14.0kDa,a single non-glycosylated polypeptide chain containing 125 amino acids.
储存条件:冷冻(-20℃)
概述
Interleukin-4(IL-4)is a pleiotropic cytokine that induces differentiation of naive helper T cells(Th0 cells)to Th2 cells and produced by mast cells,activated T cells and bone marrow stromal cells.It has many biological roles,including the stimulation of activated B-cell and T-cell proliferation,and the differentiation of CD4+ T-cells into Th2 cells.IL-4 can enhance both secretion and cell surface expression of IgE and IgG1 and also regulates the expression of the low affinity Fc receptor for IgE(CD23)on both lymphocytes and monocytes.The rat IL-4 shares about 40%~45%a.a.sequence identity with human,mouse,rhesus macaque IL-4 and they are species-specific in their activities.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
| 外观 | Sterile Filtered White lyophilized(freeze-dried)powder. |
| 纯度 | >95%by SDS-PAGE and HPLC analyses. |
| 来源 | Escherichia coli. |
| 生物活性 | Fully biologically active when compared to standard.The ED50 as determined by a cell proliferation assay using rat splenocytes is less than 1.0ng/ml,corresponding to a specific activity of>1.0×106 IU/mg. |
| 成分 | Lyophilized from a 0.2μmfiltered concentrated solution in PBS,pH 7.4,5%trehalose. |
| 内毒素 | Less than 1 EU/μg of rRtIL-4 as determined by LAL method. |
| 复溶 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/ml.Stock solutions should be apportioned into working aliquots and stored at ≤ -20?C.Further dilutions should be made in appropriate buffered solutions. |
| 氨基酸序列 | HGCNDSPLRE IINTLNQVTE KGTPCTEMFV PDVLTATRNT TENELICRAS RVLRKFYFPR DVPPCLKNKS GVLGELRKLC RGVSGLNSLR SCTVNESTLT TLKDFLESLK SILRGKYLQS CTSMS |
我公司销售的重组大鼠IL-4优惠促销,Recombinant Rat Interleukin-4,rrInterleukin-4,IL-4, Rat,rInterleukin-4,重组大鼠Interleukin-4,重组Interleukin-4质量上佳,价格普惠,欢迎广大新老客户踊跃订购。
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文献和实验图上查出相应 IL-4 含量。 试剂盒性能 1. 灵敏度 : 最小的 IL-4 检测浓度小于 16pg/ml 。 2. 特异性: 可同时检测重组或天然的大鼠 IL-4 。 不与 大鼠其它细胞因子有交叉反应。 3. 重复性:板内、板见变异系数均小于10 %。 注意事项 1. 以上标准孔及待测样品均建议做复孔,每次测定应同时做标准曲线。
1100 96孔可拆式酶标板(高吸附) 50元/块 AR1103 ELISA用ABC 140元/瓶(130μι) AR1104 Tetramethylbenzidine(TMB) 80元/瓶(10ml) AR1105 终止液(0.5M H2SO4) 30元/瓶(10ml) AR1106 样品稀释液 40元/瓶(30ml) AR1107 ELISA用生物素化抗体 380/瓶(130μι) 好消息!!! 在7月9日到9月10日期间进行暑假期间让利优惠活动,所有ELISA试剂盒7折上市促销
人的IL-2(10 ng/ml,目录号202-IL-010)和IL-4(10 ng/ml,目录号204-IL-005)的培养基培养两天;细胞洗涤后在含有重组人IL-2和IL-4的培养基中继续培养2天;最后收获细胞,使用PMA(10 ng/ml) 和Ionomycin (1 uM)刺激6小时20mL于Falcon管中,加入100mL激活后的全血(细胞浓度维持在2×106/mL) 混匀,室温暗处孵育15分钟;方法5: CD4 T 细胞使用PHA (10 ng/ml) 刺激4 days方法6: T 细胞使用
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