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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Concanavalin A
- 规格:
5mg
Overview
Con A recognizes α-linked mannose present as part of a “core oligosaccharide” in many serum and membrane glycoproteins.
Biotinylated Concanavalin A has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.
| Cat. No. | B-1005-5 |
|---|---|
| Unit Size | 5 mg |
| Country of Manufacture | United States |
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology |
| Conjugate | Biotinylated |
| Sugar Specificity | Mannose, Glucose |
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文献和实验Plasma Membrane Isolation Using Immobilized Concanavalin A Magnetic Beads
and could be developed for the isolation of organelles. We have developed a simple method for isolating plasma membranes using lectin concanavalin A (ConA) magnetic beads. ConA is immobilized onto magnetic beads by binding biotinylated ConA to streptavidin magnetic beads
[2] ✦ CUT&RUN 原理: 图 4:CUT&RUN 原理 [3] 利用连有刀豆蛋白 A 的磁珠(concanavalin A-coated magnetic beads)结合细胞。使用非离子去污剂洋地黄皂苷进行细胞膜通透。然后孵育靶蛋白(如转录因子, TF )的抗体和 Protein A-MNase 。抗体和 Protein A-MNase 能够通过核孔进入细胞核,MNase 通过 Protein A 和抗体的介导切割靶蛋白附近的 DNA 序列。 MNase 的活化需要 Ca
&Tag 技术混淆,CUT&Tag 技术在异染色质位点上有很好的结果,Henikoff 推荐的阳性对照就是存在于异染色质区域的 H3K27me3,且我们的客户在 H3K27me3 以及 H3K27AC 上都有很好的结果。 4. ConA beads 的作用是什么?如果没有 ConA beads ,能否用离心法代替? ConA beads 是经过 Concanavalin A(刀豆蛋白A,ConA)包被的磁珠。ConA 是一种从刀豆和豌豆等籽粒中提取出来的植物
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