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- 保存条件：
Powder:2-8℃，2 years;Insolvent(母液):-20℃，6 months;-80℃，1 year
- 保质期：
Powder:2-8℃，2 years;Insolvent(母液):-20℃，6 months;-80℃，1 year
- 英文名：
Bovine Serum Albumin
- 库存：
现货
- 供应商：
北京索莱宝科技有限公司
- CAS号：
9048-46-8
- 规格：
5g/1g/200mg
| 规格： | 5g | 产品价格： | ￥720.0 |
|---|---|---|---|
| 规格： | 1g | 产品价格： | ￥450.0 |
| 规格： | 200mg | 产品价格： | ￥280.0 |
是牛血清中的一种球蛋白，可用于多种生物化学实验。一般做为稳定剂被用于限制酶或者修饰酶的保存溶液和反应液中，还能防止酶吸附到管壁而损失。在PCR体系中有助于Taq酶的稳定性及活性，可以提高PCR的效率
| 基本信息 | |
| CAS | No.9048-46-8 |
| 中文名称 | 牛血清白蛋白Ⅴ |
| 英文名称 | Albumin Bovine Ⅴ |
| 别名 | BSA;Bovine albumin;白蛋白;Bovine Serum Albumin; |
| 分子量 | ≈66kDa |
| 溶解性 | Soluble in Water/D-PBS/0.85%NaCl ≥100mg/mL |
| 纯度 | HPLC≥98% |
| 外观(性状) | White to yellow Solid |
| 储存条件 | Powder:2-8℃，2 years;Insolvent(母液):-20℃，6 months;-80℃，1 year |
| EC | EINECS 232-936-2 |
| MDL | MFCD00131855 |
| 靶点 | Others |
| 通路 | Others |
| 背景说明 | 是牛血清中的一种球蛋白,可用于多种生物化学实验。一般做为稳定剂被用于限制酶或者修饰酶的保存溶液和反应液中,还能防止酶吸附到管壁而损失。 |
| 生物活性 | Bovine Serum Albumin (BSA) 是由三个同源全 α 结构域组成的 583 个氨基酸的蛋白质。BSA 是一种球状蛋白，可用于多种生物化学实验。[1] |
| In Vitro | 牛血清白蛋白(BSA)是由三个同源的全α结构域组成的583残基蛋白，以心形结构组织。牛血清白蛋白构成ca.所有血浆蛋白的60％并且结合并运输大量生理和非生理配体。牛血清白蛋白含有17个二硫键和一个未配对的半胱氨酸(Cys34)，其促进二聚化并且还影响高级缔合，因为如果Cys34与另一种化合物共价结合，则聚集速率减慢。用于原纤维细胞毒性的MTT测定显示牛血清白蛋白有利于细胞生长，而不管其聚集状态。通过膜透化测定证实缺乏细胞毒性。在随后的40小时中，观察到处理细胞的存活率增加300-400％，表明与牛血清白蛋白一起孵育的细胞比未处理的细胞具有更高的存活率[1]。 |
| 细胞实验 | 在96孔微量培养板中制备样品，并且所有不含样品的孔用200μLPBS填充以使蒸发最小化。向每个孔加载100μLSH-SY5Y生长培养基，含或不含10.000 SH-SY5Y神经母细胞瘤细胞。通过添加25μL缓冲液开始测定，其中有或没有5mg/mL超声处理的牛血清白蛋白，其预先在70℃温育0，2或96小时(给出最终BSA浓度为1mg/mL)。将所有样品组合装入三联体中，并且对于六种牛血清白蛋白组合中的每一种，加载相应的三联体而不含细胞，用作空白。一个三联体也没有细胞和BSA，只有一个没有牛血清白蛋白[1]。 |
| 数据来源文献 | [1]. Holm NK， et al. Aggregation and fibrillation of bovine serum albumin. Biochim Biophys Acta. 2007 Sep;1774(9):1128-38 |
| 单位 | 支 |
In Vitro
Cell（TNBC cell lines MDA-MB-231 and HCC1937，3% bovine serum albumin (BSA)）
The samples were blocked with 3% bovine serum albumin (BSA) (Solarbio) and probed overnight with primary antibodies against CMTM6 (1:1000), c-MYC (1:2000) and β-actin(1:5000), and with secondary antibody horseradish peroxidase-labelled IgG (1:8000) for 2 h.
来源文献：Wang X, Wang X, Gu J, Wei Y, Wang Y. circUBR5 promotes ribosome biogenesis and induces docetaxel resistance in triple-negative breast cancer cell lines via the miR-340-5p/CMTM6/c-MYC axis. Neoplasia. 2025 Jan;59:101062. doi: 10.1016/j.neo.2024.101062. Epub 2024 Dec 12. PMID: 39672097; PMCID: PMC11697786.
Cell（Neutrophils，5 % BSA for 1 h）
Neutrophils were fixed with 4 % parafor-maldehyde for 20 min,washed twice with PBS, and permeabilized with 0.1 % Triton X-100 for15 min. Neutrophils were then blocked with 5 % BSA for 1 h and incubated with anti-MPO antibody (1:100 dilution) before being incubated overnight with anti-CitH3 antibody (1:100 dilution) at 4℃.
来源文献：Nong Q, Wu Y, Liu S, Tang Y, Wu J, Huang H, Hong J, Qin Y, Xu R, Zhao W, Chen B, Huang Z, Hu L, Zhao N, Huang Y. Lead-induced actin polymerization aggravates neutrophil extracellular trap formation and contributes to vascular inflammation. Ecotoxicol Environ Saf. 2025 Jan 15;290:117598. doi: 10.1016/j.ecoenv.2024.117598. Epub 2024 Dec 24. PMID: 39721424.
Cell（ESCA cells，5 % bovine serum albumin (BSA)）
Cells on the coverslips after treatments were fixed by 4 % parafor-maldehyde for 15 min, permeabilized using 0.3 % Triton X-100(Solarbio, Beijing, China) for 10 min, and blocked in 5 % bovine serum albumin (BSA) (Solarbio, Beijing, China) in PBS for 45 min. After careful washing with PBS, the cells were incubated with primary antibodies against γ-H2AX （1:200 dilutions）, p53-binding protein 1 (53BP1) （1:200 dilutions), USP14(1:200 dilutions) and YAP1 (1:200 dilutions) at 4?C overnight. After rinsing with PBS, the cells were incubated with a fluorescence-conjugated secondary antibodies, including Alexa Fluor 647-Labeled Goat Anti-Mouse IgG (1:200 dilutions) and Alexa Fluor 488-Labeled Goat Anti-Rabbit IgG (1:200 dilutions). After nuclear staining with DAPI (Solarbio, Beijing, China), images were captured using a confocal laser-scanning microscope . Fluorescence density was quantified by use of the Image J 1.48 V software.
来源：Yuan F, Xu J, Xuan L, Deng C, Wang W, Yang R. USP14 inhibition by degrasyn induces YAP1 degradation and suppresses the progression of radioresistant esophageal cancer. Neoplasia. 2025 Feb;60:101101. doi: 10.1016/j.neo.2024.101101. Epub 2024 Dec 14. PMID: 39675091; PMCID: PMC11699344.
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AuthorYuhan Hu, Yuan Wang, Jie Deng, Xiangyu Ding, Deqing Lin, Hui Shi, Lin Chen, Dan Lin, Yuqin Wang, Serhii Vakal, Jiaqing Wang, Xingyi Li |
Publish_toJOURNAL OF CONTROLLED RELEASE |
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IF11.4670 |
PMID35278493 |
Bladder cancer selective chemotherapy with potent NQO1 substrate co-loaded prodrug nanoparticles
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AuthorBinbin Jiao, Kunpeng Liu, Haitao Gong, Zhenshan Ding, Xin Xu, Jian Ren, Guan Zhang, Qingsong Yu, Zhihua Gan |
Publish_toJOURNAL OF CONTROLLED RELEASE |
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IF11.4670 |
PMID35618186 |
Gut microbiota-derived indole-3-propionic acid alleviates diabetic kidney disease through its mitochondrial protective effect via reducing ubiquitination mediated-degradation of SIRT1
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AuthorYan Zeng, Man Guo, Qi Wu, Xiaozhen Tan, Chunxia Jiang, Fangyuan Teng, Jiao Chen, Fanjie Zhang, Xiumei Ma, Xinyue Li, Junling Gu, Wei Huang, Chunxiang Zhang, Betty Yuen-Kwan Law, Yang Long, Yong Xu |
Publish_toJournal of Advanced Research |
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IF11.4000 |
PMID39147198 |
Hypoxia-induced degradation of FTO promotes apoptosis by unmasking RACK1-mediated activation of MTK1-JNK1/2 pathway
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AuthorChengyu Li, Zhaojun Liu, Chen Fu, Hongmin Li, Tong He, Gang Wu, Yanan Sheng, Ming Shen, Honglin Liu |
Publish_toJournal of Advanced Research |
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IF11.4000 |
PMID39805423 |
Phosphatidate phosphatase Lipin1 involves in diabetic encephalopathy pathogenesis via regulating synaptic mitochondrial dynamics
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AuthorXiaolin Han, Shan Huang, Ziyun Zhuang, Xiaochen Zhang, Min Xie, Nengjun Lou, Mengyu Hua, Xianghua Zhuang, Shuyan Yu, Shihong Chen |
Publish_toRedox Biology |
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IF11.4000 |
PMID38103341 |
Overlooked Cytotoxicity and Genotoxicity to Mammalian Cells Caused by the Oxidant Peroxymonosulfate during Wastewater Treatment Compared with the Sulfate Radical-Based Ultraviolet/Peroxymonosulfate Process
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AuthorYe Du, Wen-Long Wang, Zhi-Wei Wang, Chang-Jie Yuan, Ming-Qi Ye, Qian-Yuan Wu |
Publish_toENVIRONMENTAL SCIENCE & TECHNOLOGY |
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IF11.4000 |
PMID36787277 |
Severe fever with thrombocytopenia syndrome virus induces platelet activation and apoptosis via a reactive oxygen species-dependent pathway
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AuthorYi-Hui Li, Xue-Hui Wang, Wen-Wu Huang, Ren-Rong Tian, Wei Pang, Yong-Tang Zheng |
Publish_toRedox Biology |
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IF11.4000 |
PMID37544244 |
Planting Anion Channels in a Negatively Charged Polyamide Layer for Highly Selective Nanofiltration Separation
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AuthorYuling Ren, Pengfei Qi, Yinhua Wan, Chulong Chen, Xiangrong Chen, Shichao Feng, Jianquan Luo |
Publish_toENVIRONMENTAL SCIENCE & TECHNOLOGY |
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IF11.3570 |
PMID36445263 |
CD46 分子 CD46 常用单克隆抗体或代号： HULYM5,J48 主要表达细胞： 广泛,Leu,Pt [NL] 分子质量(kDa)和结构： gp56- 66,膜辅助因子蛋白(MCP) 功 能： 调节补体活化,裂解C3b ,C4b膜辅助因子蛋白 CD46 Aka membrane cofactor protein (MCP) Cofactor for Factor
【求助】质粒有两个复制起始点，怎么复制呢？（red重组，pKD46质粒）
mnidr 我现在在做基因敲除，用的是red重组系统，pKD46质粒上携带有敲除所需的蛋白。pKD46质粒上有两个复制起始点Origin of Replication：repA101ts & oriR101 ；repA101ts是温度敏感型复制起始点。 有几个问题请教： 1.大家有用过这个pKD46的吗？oriR101是温度敏感型复制子还是条件诱导型复制子啊，我在网上查到它是温度敏感型复制起始点，我很不理解，为什么已经有一个温度
网络 四、膜辅蛋白（CD46） 膜辅蛋白（membrane cofactor protein,MCP）由Cole等（1985）应用C3b亲和层析在外周血淋巴细胞上发现的一种膜蛋白。由于其奇特的电泳特征，起初被命名为gp45-70，但进一步研究发现，它对I因子介导的对C3b和C4b的裂解有辅助活性，故更命为MCP。鉴于MCP
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