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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京索莱宝科技有限公司
- 英文名:
MCCOY’S 5A,with L-Glutamine
- 规格:
500ml/10*500ml
| 规格: | 500ml | 产品价格: | ¥80.00 |
|---|---|---|---|
| 规格: | 10*500ml | 产品价格: | ¥600.00 |
运输温度:常温运输
产品简介:1959, McCoy等报道了体外培养Novikoff Hepatoma细胞所需的氨基酸。他们在基本培养基5A的基础上通过调整加入氨基酸的量产生了MCCOY'S 5A的配方。随后Hsu 和Kellogg 使用这种培养基成功培养了一系列来源于多种组织的原代细胞,这些组织包括正常骨髓(bone marrow)、皮肤(skin)、牙龈(gingiva)、 睾丸(testes)、小鼠肾脏(mouse kidney)、网膜(omentum)、 肾上腺(adrenal glands)、肺(lung)、脾脏(spleen)、大鼠胚胎(rat embryos)及其它组织。
产品类型:无菌过滤即用型液体培养基
渗 透 压:280-330 mOsm/kg
pH:7.0-7.4
参考文献:
1. McCoy, T.A., Maxwell, M. and Kruse, P.F., (1959). Amino Acid Requirement of the Novioff Hepatoma In Vitro. Proc. Soc. Exp. Biol. Med. 100, 115-118.
2. Patterson, M.K. and Dell’orco, R.T., (1978). Preparation of McCoy’s Medium 5A. Tissue Culture Association Manual. 4, 737-740.
3. Hsu, T.C. and Kellogg, D.S., (1960). Primary Cultivation and Continuous Propagation In Vitro of Tissues from Small Biopsy Specimens. J.N.C.I. 25, 221-231.
4. Iwakata, S. and Grace, J.T., (1964). Cultivation in Vitro of Myeoblasts from Human Leukemia. New York State Journal of Med. 64, 2279-2282.
5. Morton, H.J., (1970). A Survey of Commercially Available Tissue Culture Media. In Vitro. 6, 89.

货号:M9420
样本:HEC-1-A细胞
图注:
HEC-1-A细胞镜下状态图
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文献和实验Lymphocyte Transformation 淋巴细胞转染
, each cell line requires several more weeks to reach a cell density of 1 X 108 cells/100 ml.Procedure:Collect 27 ml of anticoagulated blood in 3 yellow top tubes (citrate), 9 ml each. The blood should be set up in culture as soon as possible for best results
Dorsal Root Ganglion Neuron Culture from Adult Rats
Reagents - Euthanasia Drugs (10:3 Ketamine-Rompun or Phenobarbital) - Rat-tail collagen - Calf serum - 2.5% collagenase - 0.5% trypsin - Neuralbasal-A media (Gibco) - B-27 with antioxidants - L-glutamine - Pen-strep-neo - OPTIONAL: NGF
Method: Lymphocyte Transformation
: 2-2.5 hours to prepare two transformations. Cell lines will require 3-4 weeksin a T-25cm2 flask before passaging to a T-75cm2. After passaging to the larger flask, each cell line requires several more weeks to reach a cell density of 1 X 108
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