Phorbol 12-myristate 13-acetat

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Phorbol 12-myristate 13-acetate

CAS No. : 16561-29-8

MCE 国际站：Phorbol 12-myristate 13-acetate

产品活性：Phorbol 12-myristate 13-acetate (PMA) 是一种佛波酯，是蛋白激酶 C (PKC) 和 SphK 的激活剂。Phorbol 12-myristate 13-acetate 是 NF-κB 激活剂。Phorbol 12-myristate 13-acetate 可诱导 THP1 细胞分化。

研究领域：Epigenetics  |  TGF-beta/Smad  |  Immunology/Inflammation  |  NF-κB

作用靶点：PKC  |  SphK  |  NF-κB

In Vitro: In order to examine the role of PKC in p38MAPK phosphorylation, the cells are stimulated with the PKC activator, Phorbol 12-myristate 13-acetate (PMA) (100 nM), which mimics the binding of DAG, the natural activator of PKC, to the C1 region of the PKCs. p38MAPK phosphorylation by PMA is observed in the two cell types similar to that observed by GnRH in αT3-1 cells, that is, a slow sustained activation (3.2-fold and 3.6-fold, respectively at 30 min). The paradoxical findings that PKCs activated by GnRH and PMA play a differential role in p38MAPK phosphorylation may be explained by differential localization of the PKCs. Basal, GnRH- and PMA- stimulation of p38MAPK phosphorylation in αT3-1 cells is mediated by Ca²⁺ influx via voltage-gated Ca²⁺ channels and Ca²⁺ mobilization, while in the differentiated LβT2 gonadotrope cells it is mediated only by Ca²⁺ mobilization.
THP-1 cells are differentiated into macrophage-like cells (THP-1 macrophages) by incubation in the presence of PMA (200 ng/mL; 1-5 days), which leads to a macrophage-like phenotype characterized by changes in morphology and increased cell surface expression of CD11 and CD14.
In the monocytic cell line THP-1, PMA results in a more differentiated phenotype than VD3, according to adherence, loss of proliferation, phagocytosis of latex beads, and expression of CD11b and CD14.

In Vivo: Phorbol 12-myristate 13-acetate can be used in animal modeling to construct eczema-like models.

Phorbol 12-myristate 13-acetate (PMA) is a PKC agonist, which reverses the damage induced by 5-hydroxydecanoic acid (5-HD). Thus, activation of the mitoKATP protected mitochondrial function in SOD and MDA via the PKC pathway.

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