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- 文献和实验
- 技术资料
- 保存条件:
Powder: -20°C, 3 years; 4°C, 2 years.In solvent: -80°C, 6 months; -20°C, 1 month.
- 库存:
货期:1-2天
- 供应商:
MedChemExpress LLC
- CAS号:
313254-94-3
- 规格:
10 mM * 1 mL/5 mg/10 mg/50 mg
| 规格: | 10 mM * 1 mL | 产品价格: | ¥1030.0 |
|---|---|---|---|
| 规格: | 5 mg | 产品价格: | ¥937.0 |
| 规格: | 10 mg | 产品价格: | ¥1500.0 |
| 规格: | 50 mg | 产品价格: | ¥5800.0 |
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MMG-11
CAS No. : 313254-94-3
MCE 国际站:MMG-11
产品活性:MMG-11 是一种有效的选择性人 TLR2 拮抗剂。MMG-11 抑制 TLR2/1 和 TLR2/6 信号转导,抑制 Pam3CSK4 诱导的 hTLR2/1,IC50 为 1.7 μM,抑制 Pam2CSK4 诱导的 hTLR2/6 反应,IC50 为 5.7 μM。
作用靶点:Toll-like Receptor (TLR)
In Vitro: MMG-11 neither shows cellular toxicity nor interference with signaling induced by other TLR agonists, IL-1β or TNF. MMG-11 (0.01-100 μM) shows no cytotoxic effects up to 100 μM in peripheral blood mononuclear cells (PBMCs).
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文献和实验36 4.09 3.50 3.17 2.94 2.78 2.66 2.57 2.49 2.43 2.33 2.22 2.11 1.99 1.85 1.69 36 37 4.08 3.49 3.16 2.93 2.77 2.65 2.56 2.48 2.42 2.32 2.21 2.10 1.97 1.84 1.67 37 38 4.07 3.48 3.14 2.92 2.76 2.64 2.55 2.47 2.41 2.31 2.20 2.09 1.96 1.82 1.66 38
Single tube confirmation PCR protocol
. For example, add 5 µl of the Zymo solution from isolate #1 to PCR tubes 1-5, 5 µl of the Zymo solution from isolate #2 to PCR tubes 6-10 and so on. - Make a PCR master mix by combining: 638 µl water, 110 µl 10 x Taq Buffer, 11 µl NTP's, and 11 µl Taq
-32P]dNTP或DIG-11-dUTP,所增殖的DNA即被32P或DIG所标定,所以PCR是制备核酸探针非常便捷好用的方法。仪器用具:微量离心机;PCR 反应器药品试剂:模版DNA: pBlueGus 质体DNA (~50 pg)核酸引子对: T3 promoter primer 与T7 promoter primer (各2 μM)矿物油 (是否需要视PCR反应器机型而定)PCR DIG probe synthesis kit (Roche), 内含:Taq DNA polymerase
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