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- 2025年07月05日
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文献和实验Sephadex G-50 spun column purification
Sephadex G-50 spun column purification Spin column purification can be used to change buffers without a concomitant change in solution volume, to remove protein contaminants or to purify plasmid DNA suitable for sequencing
Sephadex G-50 spun column purificati
the syringe barrel into a 10ml centrifuge tube and spin at 3,000g for 5 minutes in a MSE Centaur with swingout rotor. 3) After centrifugation, 100-200ul of the required buffer is added to the top of the column and the column re-centrifuged. Repeat 3 times
Sephadex G-50 spun column purification
Sephadex G-50 spun column purification Spin column purification can be used to change buffers without a concomitant change in solution volume, to remove protein contaminants or to purify plasmid DNA suitable for sequencing. 1) Plug a 1
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