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- 2025年07月11日
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文献和实验Restriction Enzyme Buffer Most enzymes can use REact buffers; however, some are made up separately. Use fresh Milli-Q water , siliconized or sterile glassware or disposable plastic ware to make the following stock solutions up fresh (discard
自然染色质免疫沉淀实验设计（Native Chromatin Immunoprecipitation (N-ChIP)）
. 4.4.Resuspend the Sepharose pellet in 1ml buffer A and layer onto 9ml of the same buffer using a siliconised pasteurpipette and siliconized 15 ml tube. 4.5.Centrifuge samples (10,000 g, 10 min, 4°C), discard the S/N and wash the Sepharose sequentially in 10
IPTG Stock KCl Stock Solution, 2 M LB Agar LB Medium Lower Tris Buffer, 4x Magnesium Chloride Stock Solution MEM NaCl Stock, 5 M NaOH Stock, 10 N NCTC (medium for RCHO-1 cells) NZY
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