重组人表皮生长因子（EGF）

重组人表皮生长因子（EGF）PRP100159

产品基本信息

• 英文名称：Human EGF protein
• 种属来源：人源（Homo sapiens）
• 表达系统：大肠杆菌表达系统
• 分子量：6.3 kDa（理论值）
• 蛋白纯度：＞95%（SDS-PAGE检测）
• 产品形式：冻干粉（PBS缓冲液，pH 7.4）

关键生物活性验证

经Balb/C 3T3小鼠胚胎成纤维细胞增殖试验验证，ED₅₀为0.02-0.2 ng/mL，符合细胞培养级应用标准。

产品特性

• 无标签设计，保持天然蛋白构象
• 完整氨基酸序列（NP_001954.2，Asn971-Arg1023）
• 配套复溶缓冲液建议浓度≥100 µg/mL
• UniProt编号：P01133

保存与操作指南

• 长期保存：-20℃以下干燥环境（未复溶状态）
• 复溶后保存：
  • 4℃短期保存：2-7天
  • -20℃长期保存：建议添加0.1% HSA/BSA载体蛋白
• 操作提示：开盖前离心处理，避免反复冻融

规格与交付

100 μg | 货期：5个工作日内（期货） | 运输条件：常温运输

分子机制参考

通过EGFR受体二聚化激活下游信号通路，文献验证可调控myc、fos等增殖相关基因表达（依据《斯。诺美-走在生物医学美容最前沿》A10）。

重要声明

研究用途产品，不适用于临床诊断。使用前请查阅相关专利许可，使用者须自行承担应用合规性责任。

```

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human Epidermal Growth Factor (EGF) PRP100159

Product Basic Information

• English Name: Human EGF protein
• Species Origin: Human (Homo sapiens)
• Expression System: E. coli expression system
• Molecular Weight: 6.3 kDa (theoretical)
• Protein Purity: >95% (SDS-PAGE analysis)
• Product Form: Lyophilized powder (PBS buffer, pH 7.4)

Key Bioactivity Validation

Validated by Balb/C 3T3 mouse embryonic fibroblast proliferation assay with ED<sub>50</sub> of 0.02-0.2 ng/mL, meeting cell culture-grade application standards.

Product Characteristics

• Tag-free design maintains native protein conformation
• Complete amino acid sequence (NP_001954.2, Asn971-Arg1023)
• Recommended reconstitution buffer concentration ≥100 µg/mL
• UniProt ID: P01133

Storage & Handling Guidelines

• Long-term Storage: Below -20°C in dry environment (unreconstituted state)
• Post-reconstitution Storage:
  • Short-term at 4°C: 2-7 days
  • Long-term at -20°C: Recommended addition of 0.1% HSA/BSA carrier protein
• Handling Note: Centrifuge before opening, avoid repeated freeze-thaw cycles

Specifications & Delivery

100 μg | Lead Time: 5 working days (futures) | Shipping Conditions: Ambient temperature

Molecular Mechanism Reference

Activates downstream signaling pathways through EGFR receptor dimerization, with literature-verified regulation of proliferation-related genes including myc and fos (per "S.Novemé - At the Forefront of Biomedical Aesthetics" A10).

Important Statement

For research use only, not for clinical diagnosis. Please review relevant patent licenses before use. Users assume full responsibility for application compliance.

Note: The above information may be subject to changes due to product optimization and upgrades. For specific details, please consult AcroBiosystems.