IGF-II细胞因子,重组小鼠胰岛素样生长因子Ⅱ,重组蛋白,

Mouse IGF-II Protein, His tag (Animal-Free)

货号：PRP1191 | 产品类型：重组细胞因子

产品概述

重组小鼠胰岛素样生长因子Ⅱ（Mouse IGF-II Protein, His tag）是一种通过大肠杆菌表达系统制备的高纯度重组蛋白。该蛋白的氨基酸序列来源于小鼠胰岛素样生长因子Ⅱ（Ala25-Glu91），不含动物源成分，适用于多种细胞培养及相关体外研究应用。

关键特性

• 高纯度：经SDS-PAGE检测，纯度 > 98%
• 低内毒素：LAL法检测，内毒素含量 < 0.1 EU/μg
• 高生物活性：ED₅₀测定显示其诱导MCF-7细胞增殖能力显著，比活性 > 1.5 × 10⁵ IU/mg
• 无动物源表达：采用Animal-Free生产工艺，降低外源因子风险

技术参数

种属	小鼠（Mouse）
表达系统	大肠杆菌（E.coli）
分子量	约 11 kDa
标签	无（Null）
蛋白形态	冻干粉（冻干缓冲液：无菌PBS, pH 8.0）

应用建议

本品推荐用于细胞增殖、分化、迁移与存活等相关机制研究，尤其适用于胚胎发育、肿瘤生长等领域的体外细胞模型。

使用注意：

• 使用前请离心，避免粉末散失
• 推荐使用配套复溶缓冲液复溶，浓度不低于 100 µg/mL
• 复溶后可依据实验需求进一步稀释

保存与运输

保存条件：

• 冻干粉可在 -20°C 下稳定保存1年
• 复溶后在 2°C–8°C 下可保存1周
• 稀释后分装保存于 -20°C 或 -80°C，避免反复冻融

运输条件：蓝冰运输，确保蛋白稳定性

背景信息

小鼠胰岛素样生长因子Ⅱ（IGF-II）是一种由67个氨基酸组成、分子量约7.34 kDa的生长因子，主要在胚胎发育期体细胞组织、肝脏及大脑上皮细胞中表达。IGF-II通过结合IGF-I受体参与调控细胞增殖、迁移、分化和存活，在胚胎发育、胎盘形成及肿瘤发生中具有重要作用。

产品规格与价格

规格	价格（元）	货期
5 µg	588	请咨询
20 µg	1498	请咨询
100 µg	3989	请咨询
1 mg	请咨询	请咨询

警告：本品仅供科研使用，不适用于人体或临床诊断。用户须自行确保应用不侵犯专利或许可证限制。

别名：AL033362, Igf-2, M6pr, Mpr, Peg, Peg2

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mouse IGF‑II Protein, His tag (Animal‑Free)

Item No.: PRP1191 | Product Type: Recombinant cytokine

Product Overview

The recombinant mouse insulin‑like growth factor‑II (Mouse IGF‑II Protein, His tag) is a high‑purity recombinant protein produced in an E. coli expression system. The amino‑acid sequence corresponds to mouse IGF‑II (Ala25‑Glu91), contains no animal‑derived components, and is suitable for a variety of cell‑culture and related in vitro research applications.

Key Features

• High Purity: > 98 % purity confirmed by SDS‑PAGE
• Low Endotoxin: < 0.1 EU/µg as measured by LAL assay
• High Biological Activity: ED₅₀ assay demonstrates potent induction of MCF‑7 proliferation; specific activity > 1.5 × 10⁵ IU/mg
• Animal‑Free Expression: Produced using an animal‑free process to minimize exogenous factor risk

Technical Specifications

Species	Mouse
Expression System	E. coli
Molecular Weight	≈ 11 kDa
Tag	None (Null)
Formulation	Lyophilized powder (lyophilization buffer: sterile PBS, pH 8.0)

Suggested Applications

Recommended for studies of cell proliferation, differentiation, migration, and survival, particularly in vitro cellular models of embryonic development and tumor growth.

Usage Notes:

• Centrifuge before use to avoid loss of powder
• Reconstitute with the provided buffer to a concentration ≥ 100 µg/mL
• Further dilute as required by experimental protocols after reconstitution

Storage and Shipping

Storage Conditions:

• Lyophilized powder stable at –20 °C for up to 1 year
• Reconstituted solution stable at 2–8 °C for 1 week
• Aliquoted diluted samples stored at –20 °C or –80 °C; avoid repeated freeze‑thaw cycles

Shipping Conditions: Shipped on dry ice to maintain protein stability

Background Information

Mouse insulin‑like growth factor‑II (IGF‑II) is a 67‑amino‑acid growth factor (~7.34 kDa) predominantly expressed in somatic tissues, liver, and brain epithelial cells during embryogenesis. IGF‑II binds the IGF‑I receptor to regulate cell proliferation, migration, differentiation, and survival, playing critical roles in embryonic development, placenta formation, and tumorigenesis.

Product Specifications and Pricing

Specification	Price (CNY)	Lead Time
5 µg	588	Please inquire
20 µg	1498	Please inquire
100 µg	3989	Please inquire
1 mg	Please inquire	Please inquire

Warning: For research use only; not intended for human or clinical diagnostic applications. Users must ensure that their use does not infringe any patents or licensing restrictions.

Synonyms: AL033362, Igf-2, M6pr, Mpr, Peg, Peg2

Note: The above information is subject to change due to product optimization or upgrades; please contact Yakoin for the most current details.