- ¥1440
- BOSSBIO
- H-0016-B
- USA
- 2025年11月25日
- HC-P IHC WB ELISA IC
- human
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
BOSSBIO
- 应用范围：
HC-P IHC WB ELISA IC
- 宿主：
human
- 库存：
大量
- 抗原来源：
human
- 保质期：
一年以上
- 供应商：
博胜经纬
- 级别：
免疫球蛋白
- 是否单克隆：
多克隆
- 保存条件：
-20℃
- 规格：
5mg/10mg
Human IgA （ Immunoglobulines Antigen )
Quantity size: 20mg ( 20mg/ML ) packaging: Liquid form
Host species： human
Product type : Immunoglobulines Antigen - human IgA
Purification Method: Purified by Protein-A affinity chromatography. purity :>90%
Tested applications: · Use for biotechnology
Dilution method : pH 7.2 0.01 M PBS or Sterile water dilution
Please Note: Optimal dilutions should be determined by each laboratory for each application.
Storage instructions: Store at 2-4 ºC short term ( 4-6 Month ). Aliquot and store at -20ºC long term( 1-2 Years). Avoid repeated freeze / thaw cycles.
Background:
IgA (immunoglobulin A) is a glycosylated protein of 160 kDa and is produced as a monomer or as a J chain linked dimer. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA..
.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
人血IgA 制品中文说明介绍
材 料：正常人血清
纯化方法：用去人IgM后的人血清作原料，EDTA沉淀去除杂蛋白，再经HPLC纯化二次制成IgA蛋白液体。
分析与鉴定：
1.特异分析，对羊抗人IgA免疫血清双扩出一条沉淀线，对羊抗人IgG产生一条很弱沉淀线，对羊抗人血清双扩出一条IgA、一条IgG（弱沉淀线）。
2.分子量测定：SDS-PAGA分子量测定为16万左右蛋白带。
3.蛋白浓度测定：以IgA蛋白的紫外光280nm的消光系数为：1.34计算，IgA（mg/ml）=OD280nm吸收值/1.34×稀释倍数。
性状为无色透明液体，浓度为：10mg/ml
北京博胜经纬科技有限公司所提供的IgA免疫球蛋白抗原，均由我公司数名海外归国从事多年生命科学研究领域的资深免疫学及化学专家和科研工作者，通过多年不断的努力，积累国内外大量重要科研文献及蛋白提纯技术资料，总结经验、不断改良，反复试验而制成。产品已经通过我公司严格的质量出产控制，纯度在90%左右。本公司自建的技术已经达到国际化水准。该产品敢与国外产品相媲美。
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文献和实验Purification and Characterization of Human Serum and Secretory IgA1 and IgA2 Using Jacalin
Human immunoglobulin A (IgA) is found in serum and secretions in several different forms (1 ), Serum IgA is produced by the bone marrow and is pre-dominately monomeric (M r = 160 kDa
Immunoassay for Detection of IgA Antitissue Transglutaminase in Patients with Celiac Disease
under a gluten-free diet. Therefore, these antibodies are useful tools for diagnosis, and in the dietary control of coeliac disease (3 –7 ). Serum IgA antibodies against endomysial antibodies (EMAs) are especially considered to be sensitive and highly specific
Production of Human Monoclonal Antibodies by the EpsteinBarr Virus Method
. The method allows the production of human IgM, IgG, IgA, and IgE monoclonal antibodies from any individual. Monoclonal antibodies produced by the EBV method resemble the antibody repertoire of the donor of the lymphocytes. Human monoclonal antibodies
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