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        Purification and Characterization of Human Serum and Secretory IgA1 and IgA2 Using Jacalin

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        Human immunoglobulin A (IgA) is found in serum and secretions in several different forms (1 ), Serum IgA is produced by the bone marrow and is pre-dominately monomeric (M r = 160 kDa). Most of the IgA in secretions (sIgA) is polymeric, predominantly dimeric, comprising two monomer subunits linked together by intersubunit disulfide bonds and a cysteine-rich polypeptide termed J-chain (M r = 16 kDa). Polymeric IgA in secretions, but not in serum, contains an additional heavily glycosylated protein called secretory component (SC) (M r = 70 kDa).
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