M1520-57 MN-c 小鼠皮质神经元 Mouse Neurons-cortical 1 x 10^6 cells/vial
M1530-57 MGC 小鼠颗粒细胞 Mouse Granule Cells 1 x 10^6 cells/vial
M1540-57 MN-h 小鼠海马趾神经元 Mouse Neurons-hippocampal 1 x 10^6 cells/vial
M1700-57 MSC 小鼠雪旺细胞 Mouse Schwann Cells 5 x 10^5 cells/vial
M1800-57 MA 小鼠星形胶质细胞 Mouse Astrocytes 5 x 10^5 cells/vial
M1810-57 MA-c 小鼠小脑星形胶质细胞 Mouse Astrocytes-cerebellar 5 x 10^5 cells/vial
M1820-57 MA-h 小鼠海马趾星形胶质细胞 Mouse Astrocytes-hippocampal 5 x 10^5 cells/vial
M1900-57 MM 小鼠小胶质细胞 Mouse Microglia 5 x 10^5 cells/vial
M2530-57 MLF 小鼠淋巴成纤维细胞 Mouse Lymphatic Fibroblasts 5 x 10^5 cells/vial
M4100-57 MRTEpiC 小鼠肾小管上皮细胞 Mouse Renal Tubular Epithelial Cells 5 x 10^5 cells/vial
M6200-57 MCM 小鼠心肌细胞 Mouse Cardiac Myocytes 1 x 10^6 cells/vial
M7500-57 MMSC-bm 小鼠骨髓间充值干细胞 Mouse Mesenchymal Stem Cells-bone marrow 5 x 10^5 cells/vial
M7540-57 MEF-mt 小鼠胚胎成纤维细胞 Mouse Embryonic Fibroblasts 5 x 10^5 cells/vial
小鼠胚胎成纤维细胞CD57BL/6小鼠细胞说明:
货号:M7540-57
Description
Mouse Embryonic Fibroblasts (MEF) are used to support the growth of undifferentiated mouse or human ES cells and iPS cells [1]. They provide both a substrate for the ES cells to grow on and secrete many factors necessary for ES cells to maintain pluripotency. MEF cells are isolated from mouse embryos and are used at their early passages [2]. Before MEF cells are used as feeder cells, they must be treated by irradiation or mitomycin C to stop the cells from further dividing.
MEF from ScienCell Research Laboratories are isolated from day 13 mouse embryos. MEF are cryopreserved at primary culture and delivered frozen. Each vial contains 5 x 106 cells in 1 ml volume. MEF are characterized by immunofluorescentr method with antibodies to fibronectin. MEF are negative for mycoplasma, bacteria, yeast and fungi. MEF are guaranteed to further culture over 5 population doublings at the conditions provided by ScienCell Research Laboratories.
Recommended Medium It is recommended to use DMEM with 10% FBS for the culturing of MEF in vitro.
Product Use MEF are used as feeder layer in mouse and human ES or iPS cell culture. They are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.