LinKine™ AbFluor™ 680偶联试剂盒

LinKine™ AbFluor™ 680偶联试剂盒（货号：KTL0580）

核心产品定位

• 产品分类：蛋白偶联类核心试剂盒
• 应用领域：蛋白类生物分子标记，特别适用于抗体/蛋白质与近红外荧光探针的定向偶联
• 技术平台：样品制备及检测技术中心开发

创新标记体系

• 核心组分：
  • 预活化AbFluor™ 680溶液（即用型）
  • 专利偶联缓冲体系（pH 8.5±0.3）
  • 50KD分子筛纯化柱（0.5mL×3）
• 技术优势：
  • 专利活化工艺使染料摩尔吸光系数达220,000 cm⁻¹M⁻¹
  • 标准化标记流程实现F/P比值0.8-1.2最佳区间
  • 纯化柱可实现>85%蛋白回收率（2mg/mL样本验证）

操作规范要求

• 样本预处理：建议透析至PBS缓冲液（pH7.4），避免TRIS/甘氨酸干扰
• 浓度控制：标记起始浓度≥2mg/mL（超滤浓缩建议采用30KD滤膜）
• 染料保护：活化染料需避光操作，溶解后建议2小时内完成标记

物流保障体系

规格	包装内容	价格（CNY）	交付周期
3×100μg	3支活化染料+缓冲液+纯化柱套组	1689	冷链运输，订单确认后24h内发运
1mg	10支活化染料+缓冲液+纯化柱套组	2716

技术支持参数

• 光谱特性：Ex/Em=681/698nm（PBS缓冲体系实测值）
• 储存条件：-20℃避光保存，复溶后分装冻存（避免反复冻融）
• 兼容验证：流式细胞仪/小动物成像系统/激光共聚焦通用检测通道

重要声明：

本产品适用于科研用途，性能参数基于标准BSA标记实验获得，实际应用效果可能因实验条件差异而不同。使用前请查阅相关专利状态，实验操作需符合所在机构生物安全规范。

搜索关键词：AbFluor™ 680试剂盒 / Cy5.5替代染料 / 近红外蛋白标记 / KTL0580

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

LinKine™ AbFluor™ 680 Conjugation Kit (Cat. No.: KTL0580)

Core Product Positioning

• Product Category: Protein conjugation core reagent kit
• Application Field: Biomolecular labeling of proteins, particularly suitable for directional conjugation of antibodies/proteins with near-infrared fluorescent probes
• Technology Platform: Developed by the Sample Preparation and Detection Technology Center

Innovative Labeling System

• Core Components:
  • Pre-activated AbFluor™ 680 solution (ready-to-use)
  • Patented conjugation buffer system (pH 8.5±0.3)
  • 50KD molecular sieve purification columns (0.5mL×3)
• Technical Advantages:
  • Patented activation process achieves dye molar extinction coefficient of 220,000 cm⁻¹M⁻¹
  • Standardized labeling protocol achieves optimal F/P ratio of 0.8-1.2
  • Purification columns enable >85% protein recovery (verified with 2mg/mL samples)

Operational Specifications

• Sample Pretreatment: Recommended dialysis into PBS buffer (pH 7.4), avoiding TRIS/glycine interference
• Concentration Control: Labeling starting concentration ≥2mg/mL (30KD filter membrane recommended for ultrafiltration concentration)
• Dye Protection: Light-protected handling required for activated dye, complete labeling within 2 hours after dissolution

Logistics Assurance System

Specification	Package Contents	Price (CNY)	Delivery Cycle
3×100μg	3 vials of activated dye + buffer + purification column set	1689	Cold chain transportation, shipped within 24h after order confirmation
1mg	10 vials of activated dye + buffer + purification column set	2716

Technical Support Parameters

• Spectral Characteristics: Ex/Em=681/698nm (measured in PBS buffer system)
• Storage Conditions: Store at -20°C protected from light, aliquot and freeze after reconstitution (avoid repeated freeze-thaw cycles)
• Compatibility Validation: Compatible with flow cytometry/small animal imaging systems/confocal laser scanning microscopy detection channels

Important Statement:

This product is intended for research use only. Performance parameters were obtained from standard BSA labeling experiments, and actual application results may vary depending on experimental conditions. Please check relevant patent status before use. Experimental operations must comply with institutional biosafety regulations.

Search Keywords: AbFluor™ 680 Kit / Cy5.5 alternative dye / Near-infrared protein labeling / KTL0580

Note: The above information may change due to product optimization and upgrades. For specific details, please consult Yakeyin Company.