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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 供应商:
上海觅拓
- 英文名:
KnockOut™ Serum Replacement
- 规格:
100ml/500ml
| 规格: | 100ml | 产品价格: | ¥1192.0 |
|---|---|---|---|
| 规格: | 500ml | 产品价格: | ¥3550.0 |
描述
Knockout™ 血清替代物 (Knockout™ SR) 适用于对来自多个物种的胚胎干细胞 (ESC) 和诱导多能干细胞 (iPSC) 进行无血清的滋养细胞依赖性培养。这种成分明确的无血清配方可直接替代现有实验方案中的 FBS。
使用 KnockOut™ 血清替代物能够消除使用 FBS 进行干细胞培养时面临的许多缺点,包括细胞分化和热灭活。与添加 FBS 的培养基相比,在添加 KnockOut™ SR 的培养基中培育时,ES 和 iPS 细胞出现的分化显著更少,种系传递能力应该不会受到影响。在 2000 多篇涵盖干细胞培养、分化、iPSC 生成、ESC 衍生化以及转基因建模等应用的同行评审出版物中,KnockOut™ 血清替代物被广泛应用于干细胞培养。
规格
| Manufacturing Quality: | cGMP for medical devices, 21 CFR Part 820 and ISO 13485 |
|---|---|
| Classification: | Serum-Free |
| Cell Type: | Stem Cells (Embryonic) |
| Product Line: | KnockOut™ |
| Product Size: | 500 mL |
| Green Features: | Sustainable packaging |
| Shipping Condition: | Dry Ice |
内容及储存
Store in freezer (-5 to -30°C) and protect from light.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
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文献和实验Scarless Chromosomal Gene Knockout Methods
recombination proteins, which carry out the replacement of a target genomic region with a marker-containing linear DNA cassette, and (2) a rhamnose-inducible promoter that drives expression of I-Sce I endonuclease, which accomplishes deletion of the introduced
High-Throughput Production of Gene Replacement Mutants in Neurospora crassa
The model filamentous fungus Neurospora crassa has been the focus of functional genomics studies for the past several years. A high-throughput gene knockout procedure has been developed and used to generate mutants for more than two-thirds
Recombineering/Lambda red-mediated gene replacement
Overview Single-gene knockouts using λ red system, adapted from Datsenko and Wanner paper. The goal of this protocol was to create an endA (endonuclease I) knockout, but obviously it can be adapted to any gene. The knocked-out gene
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