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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原:
None
- 亚型:
IgG2a
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
human, mouse, rat
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Mouse
- 应用范围:
IHC, IF/ICC, ELISA
- 靶点:
BrdU
- 抗体英文名:
BrdU Monoclonal antibody
- 抗体名:
BrdU Monoclonal antibody
- 规格:
100ul/500ul/1ml
| 规格: | 100ul | 产品价格: | ¥980.0 |
|---|---|---|---|
| 规格: | 500ul | 产品价格: | ¥4000.0 |
| 规格: | 1ml | 产品价格: | ¥6000.0 |
经过测试的应用
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, L-929 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66241-1-Ig targets BrdU in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IHC, IF/ICC, ELISA |
| 文献引用应用 | IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, zebrafish |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | BrdU |
| 别名 | 5-bromo-2-deoxyuridine, BrdUrd, Bromodeoxyuridine, BUdR |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_2881630 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has 0mitogenic, transcriptional and translational effects on cells that incorporate it. This antibody does not cross react with Thymidine.
Protocol for IHC:
https://www.ptgcn.com/protocol/66241-1-IgIHC.pdf
Protocol for IF:
https://www.ptgcn.com/protocol/66241-1-IgIF.pdf
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- 作者
- 内容
- 询问日期
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|---|---|---|
Cell Metab The browning and mobilization of subcutaneous white adipose tissue supports efficient skin repairAuthors - Junrong Cai | ||
| mouse | IHC,IF | Nat Cell Biol LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis.Authors - Pingping Zhu |
| mouse | IF | Sci Transl Med FoxO transcription factors modulate autophagy and proteoglycan 4 in cartilage homeostasis and osteoarthritis.Authors - Tokio Matsuzaki |
| mouse | IF | Cell Res A novel m6A reader Prrc2a controls oligodendroglial specification and myelination.Authors - Rong Wu |
| human | IF | Nat Commun MRNIP condensates promote DNA double-strand break sensing and end resection.Authors - Yun-Long Wang |
Nat Commun The methyltransferase METTL3 negatively regulates nonalcoholic steatohepatitis (NASH) progression.Authors - Xinzhi Li |
摘要: 为了满足广泛开展BrdU标记DNA检测对BrdU特异性单抗的需要,我们制备了一株产生BrdU单抗的杂交瘤细胞株.本文对此细胞株制备过程及单抗鉴定情况作了简单叙述. BrdU单克隆 抗体 的制备及鉴定 中华 微生物 学和免疫学杂志 1999年第3期
抗体的检测外,还应该用与其抗原成分相关的其它抗原进行交叉试验,方法可用ELISA、IFA法。例如:①制备抗黑色素瘤细胞的McAb,除用黑色素瘤细胞反应外,还应该用其它脏器的肿瘤细胞和正常细胞进行交叉反应,以便挑选肿瘤特异性或肿瘤相关抗原的单克隆抗体。②制备抗重组的细胞因子的单克隆抗体,应首先考虑是否与表达菌株的蛋白有交叉反应,其次是与其它细胞因子间有无交叉。 2.McAb的Ig类与亚类的鉴定 一般在用酶标或荧光素标记的第二抗体进行筛选时已经基本上确定了抗体的Ig类型
免疫系统受抗原刺激后,B细胞转化为浆细胞,由浆细胞产生与抗原发生特异性结合的球蛋白,这类球蛋白称为抗体(antibody,Ab)。 免疫球蛋白(immunoglobulim,Ig)是指具有抗体活性或化学结构与抗体相似的球蛋白。 免疫球蛋白普遍存在于哺乳动物和人类的血液、组织液和外分泌中。人体的免疫球蛋白主要有五类:IgM、IgA、IgG、IgD、IgE。 (一) 抗体结构 抗体是具有4条多肽链的对称
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