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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
KLH-conjugated 5-iodouridine
- 亚型:
Mouse IgG1
- 形态:
Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4, 5 mg/ml BSA, 25% glycerol with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography.
- 保存条件:
Store at -20°C.
- 克隆性:
单克隆抗体
- 标记物:
见下方详细说明
- 适应物种:
Other
- 宿主:
Mouse
- 应用范围:
E, IHC, IF
- 浓度:
见详细说明信息
- 靶点:
BrdU
- 抗体英文名:
BrdU Antibody
- 抗体名:
BrdU Antibody
- 规格:
0.1 mg
BrdU Antibody产品详细介绍:
| 产品名称: | BrdU Antibody |
| 说明书: | 【点击查看详细说明及参考图片】 |
| 货号: | 254535 |
| 规格: | 0.1 mg |
| 产品描述: | Bromodeoxyuridine (BrdU) is used to study DNA synthesis in which BrdU is incorporated in place of thymidine. This antibody can be used to detect BrdU incorporation for immunocytochemical analysis of cell proliferation. |
| 克隆类型: | Mouse Monoclonal Antibody |
| 亚型: | Mouse IgG1 |
| 免疫原: | KLH-conjugated 5-iodouridine |
| 适用物种: | Other |
| 应用范围: | E, IHC, IF |
| 应用说明: | E: 1:500-1:1,000; IHC: 1:200-1:500; IF: 1:200-1:500 |
| 别名: | Bromodeoxyuridine; BrdU; 5-bromo-2'-deoxyuridine |
| 形态: | Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4, 5 mg/ml BSA, 25% glycerol with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography. |
| 保存条件: | Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment. |
| 参考文献: |
ABBIOTEC产品提供质量保证,提供完善的售后和技术支持,ABBIOTEC产品正品包装:
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文献和实验摘要: 为了满足广泛开展BrdU标记DNA检测对BrdU特异性单抗的需要,我们制备了一株产生BrdU单抗的杂交瘤细胞株.本文对此细胞株制备过程及单抗鉴定情况作了简单叙述. BrdU单克隆 抗体 的制备及鉴定 中华 微生物 学和免疫学杂志 1999年第3期
实验材料 Materials Required But Not Provided 1. BrdU reagent (Invitrogen Cat. No. 00-0103) 2. Cytospin Tubes 3. Phosphate-Buffered Saline 4. 0.22 micron filter 实验步骤 1. Labeling with BrdU In Vivo
Bromodeoxyuridine (BrdU) uses nucleotide substitution to replace thymidine with uridine in the DNA structure of dividing cells both in vitro and in vivo (Gage, 2000). BrdU has been utilised in a number of in vitro and in vivo studies to label
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