- ￥8977
- 原装进口/国产
- 进口
- ALX-850-270-KI01
- 2025年12月05日
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- 详细信息
- 询价记录
- 技术资料
- 库存：
3-4周
- 供应商：
上海研卉生物科技有限公司
- 应用：
elisa 体外检测
- 规格：
96T
人Apoptosense M30 ELISA试剂盒详细说明
产品中文名称：人Apoptosense M30 ELISA试剂盒产品英文名称：Human Apoptosense M30 Elisa Kit
| Assay Time: | <5 hours |
| Application: | For the specific and sensitive detection of caspase-cleaved intermediate filament protein cytokeratin-18 (CK18Asp396-NE: M30 neo-epitope). The M30-Apoptosense® ELISA is primarely intended for quantitative determination of epithelial apoptosis in cytosolic cell extracts or cell culture supernatants, human serum or plasma or other body fluids. Use in combination with the M65® ELISA Kit (Prod. No. ALX-850-310) to allow the calculation of apoptosis:necrosis ratios. |
| Handling: | Do not freeze. |
| Long Term Storage: | +4°C |
| Background / Technical Information: | Cytokeratin-18 expression has been shown in but is not limited to cells and tissues from lung, breast, intestine, gut, colon, kidney, pancreas, bladder, trophoblasts.The M30-Apoptosense® ELISA uses the well-established M30 CytoDEATH monoclonal antibody which has been shown in numerous independent studies to be specific to the CK18 Asp396 caspase cleavage site and represents a validated apoptosis marker that does not react with viable or necrotic cells. Apoptosis detection using M30 CytoDEATH has been described in several publications to serve as a superior technique compared to TUNEL or detection of gammaH2AX phosphorylation. Assessment of apoptosis by immunohistochemical markers compared to cellular morphology in ex vivo-stressed colonic mucosa under conditions of DNA-damage may be repaired and results in false-positive counts of apoptotic cells. In addition has modest or transient caspase activation without the cleavage of death substrates been attributed to non-suicidal processes such as proliferation, differentiation and inflammation. The unmatched sensitivity and superior performance characteristics of M30-Apoptosense® ELISA even allows the tracing of apoptotic epithelial cells (e.g. carcinoma cells) in human blood samples as no contribution comes from apoptotic inflammatory lymphoid cells as those belong -together with neuronal cells and tissues- to the prominent populations of Cytokeratin-18 negative cells. By measuring the caspase-cleaved Cytokeratin-18 with M30 one can selectively quantify the extent of non-lymphoid e.g. epithelial carcinoma target cells in natural killer (NK) activity tests in one well containing a mixed cell population of otherwise un-labeled effector (T cells: CK18 and M30 negative) and target cells (carcinoma cells: CK18 and M30 positive). As the principle of the M30-Apoptosense® ELISA is based upon measuring the rapid accumulation of a caspase cleavage product -that is comparably stable- rather than proteolytic activity itself, it is recommended as an advantageous assay for high throughput screening (HTS) selective for pro-apoptotic compounds: - It avoids the necessity to analyse multiple time-points after the apoptotic stimulus as it has the broadest time-window for optimal signal strength compared to alternative apoptosis detection methods as it does not rely on cell proliferation M30-Apoptosense® ELISA avoids false positive counts due to cell cycle arrest, senescence or differentiation. - For mechanistically studies the optional analysis of only the cell culture supernatant avoiding cell lysis allows the ongoing manipulation of the cells to study sensitization or synergistic effects of different stimuli (e.g. drugs) in the same tissue culture well. |
| UniProt ID: | P05783 |
Product Literature References
Apoptotic cytokeratin 18 neoepitopes in serum of patients with chronic hepatitis C: B. Kronenberger, et al.; J. Viral Hepat. 12, 307 (2005), Abstract;
Expression of the antiapoptotic gene seladin-1 and octreotide-induced apoptosis in growth hormone-secreting and nonfunctioning pituitary adenomas: P. Luciani, et al.; J. Clin. Endocrinol. Metab. 90, 6156 (2005), Abstract; Full Text
The extent of liver steatosis in chronic hepatitis C virus infection is mirrored by caspase activity in serum: N. Seidel, et al.; Hepatology 42, 113 (2005), Abstract;
Validation of pharmacodynamic assays to evaluate the clinical efficacy of an antisense compound (AEG 35156) targeted to the X-linked inhibitor of apoptosis protein XIAP: J. Cummings, et al.; Br. J. Cancer 92, 532 (2005), Abstract;
Differentiation between cell death modes using measurements of different soluble forms of extracellular cytokeratin 18: G. Kramer, et al.; Cancer Res. 64, 1751 (2004), Abstract;
Keratin 8/18 breakdown and reorganization during apoptosis: B. Schutte, et al.; Exp. Cell Res. 297, 11 (2004), Abstract;
A novel assay for discovery and characterization of pro-apoptotic drugs and for monitoring apoptosis in patient sera: K. Biven, et al.; Apoptosis 8, 263 (2003), Abstract;
Betulinic acid: a new cytotoxic compound against malignant head and neck cancer cells: D. Thurnher, et al.; Head and Neck 25, 732 (2003), Abstract;
Measurement of an apoptotic product in the sera of breast cancer patients: T. Ueno, et al.; Eur. J. Cancer 39, 769 (2003), Abstract;
A novel high-through-put assay for screening of pro-apoptotic drugs: M. Hagg, et al.; Invest. New Drugs 20, 253 (2002), Abstract;
An immunohistochemical study of the clearance of apoptotic cellular fragments: M.P. Leers, et al.; Cell. Mol. Life Sci. 59, 1358 (2002), Abstract;
Cisplatin induces the proapoptotic conformation of Bak in a deltaMEKK1-dependent manner: A. Mandic, et al.; Mol. Cell. Biol. 21, 3684 (2001), Abstract;
Immunocytochemical detection and mapping of a cytokeratin 18 neo-epitope exposed during early apoptosis: M.P. Leers, et al.; J. Pathol. 187, 567 (1999), Abstract;
For an extensive and regularly updated literature list, please click here.: (0),
人,小鼠,大鼠,猪,兔,犬，其它动物细胞因子;细胞凋亡,活性多肽、自身抗体,血栓与止血,
骨代谢,肝纤维化,肿瘤,激素内分泌、自身抗体科研ELISA检测试剂盒
产品详细情况请来电咨询
品种齐全：人，小，鼠，大鼠，豚鼠，猪，狗，牛，羊，猴，兔，等动物种属
样本齐全：可以为细胞培养上清液，血清，血浆，尿液，组织液，心房水，体液标本等
细胞因子系列检测试剂盒 肿瘤标志物系列检测试剂盒 自身免疫系列检测试剂盒
心脏病系列检测试剂盒 内分泌系列检测试剂盒 心肌梗塞系列检测试剂盒
肝纤维化系列检测试剂盒 传染病系列检测试剂盒 微生物传染病系列检测试剂盒
细胞免疫系列检测试剂盒 优生优育系列检测 特种蛋白系列检测
M65 elisa kit
M65® ELISA kit
| ALX-850-310-KI01 | 96 wells | (CE Mark) | INQUIRE | ||
Product Specification
| Assay Time: | <2.5 hours |
| Application: | For the specific and sensitive detection of soluble CK18 (cytokeratin 18). The M65® ELISA is primarily intended for quantitative determination of total cell death (apoptosis and necrosis) of CK18-positive cells (e.g. carcinoma) in human serum and plasma samples. Use in combination with the M30-Apoptosense® ELISA Kit (Prod. No. ALX-850-270) to allow the calculation of apoptosis:necrosis ratios. |
| Handling: | Do not freeze. |
| Long Term Storage: | +4°C |
| UniProt ID: | P05783 |
Product Literature References
Induction of lysosomal membrane permeabilization by compounds that activate p53-independent apoptosis: H. Erdal, et al.; PNAS 102, 192 (2005), Abstract;
Apoptosis pathway-targeted drugs--from the bench to the clinic: J. Cummings, et al; Biochim. Biophys. Acta 1705, 53 (2004), Abstract;
Determining tumor apoptosis and necrosis in patient serum using cytokeratin 18 as a biomarker: S. Linder, et al.; Cancer Lett. 214, 1 (2004), Abstract;
Differentiation between cell death modes using measurements of different soluble forms of extracellular cytokeratin 18: G. Kramer, et al.; Cancer Res. 64, 1751 (2004), Abstract;
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人Apoptosense M30 ELISA试剂盒
￥8977
