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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human CCNH protein
- 亚型:
IgG2a
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
human, mouse, rat
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Mouse
- 应用范围:
WB, IHC, IF/ICC, FC (Intra), ELISA
- 靶点:
Cyclin H
- 抗体英文名:
Cyclin H Monoclonal antibody
- 抗体名:
Cyclin H Monoclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1350.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2200.0 |
| 规格: | 150ul | 产品价格: | ¥3000.0 |
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, MCF-7 cells, NCCIT cells, HT-1080 cells, LNCaP cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-3 cells |
| Positive FC (Intra) detected in | PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67065-1-Ig targets Cyclin H in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag28556 Product name: Recombinant human CCNH protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-323 aa of BC005280 Sequence: MYHNSSQKRHWTFSSEEQLARLRADANRKFRCKAVANGKVLPNDPVFLEPHEEMTLCKYYEKRLLEFCSVFKPAMPRSVVGTACMYFKRFYLNNSVMEYHPRIIMLTCAFLACKVDEFNVSSPQFVGNLRESPLGQEKALEQILEYELLLIQQLNFHLIVHNPYRPFEGFLIDLKTRYPILENPEILRKTADDFLNRIALTDAYLLYTPSQIALTAILSSASRAGITMESYLSESLMLKENRTCLSQLLDIMKSMRNLVKKYEPPRSEEVAVLKQKLERCHSAELALNVITKKRKGYEDDDYVSKKSKHEEEEWTDDDLVESL |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | cyclin H |
| 别名 | CCNH, 2C5G11, p34, MO15-associated protein, MO15 associated protein |
| 计算分子量 | 38 kDa |
| 观测分子量 | 38 kDa |
| GenBank蛋白编号 | BC005280 |
| 基因名称 | Cyclin H |
| Gene ID (NCBI) | 902 |
| RRID | AB_2882375 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P51946 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | WB | Genes Dev TRF1 uses a noncanonical function of TFIIH to promote telomere replicationAuthors - Zhe Yang |
| mouse | WB | Adv Sci (Weinh) m6A Reader hnRNPA2B1 Modulates Late Pachytene Progression in Male Meiosis Through Post-Transcriptional ControlAuthors - Lisha Yin |
受体BCR。 天然Ig的基本结构是一Y型的四肽链。 免疫球蛋白的基本结构示意图 典型的Is分子基本结构呈“Y”字型,由两条相同的重链(H)和两条相同的轻链(1。)借二硫键连接而成。重链和轻链近氨基端的l/4或l/2氨基酸序列的变化很大,为可变区;其他部分氨基酸序列则相对恒定,为恒定区;位于CHI与CH2之间、富含脯氦酸的区域为铰链区。 Vu和VL分别代表重链和轻链的可变区,Cu和CI。分别代表重链和轻链的恒定区。
CDK Pathway: Cyclin-Dependent Kinases (cdks) and Cyclin-Dependent Kinase Inhibitors
the DNA in a population of cells with propidium iodide and then determining the cell-cycle profile of that population by fluorescence-activated cell sorter (FACS) or by measuring 3 H-thymidine incorporation into DNA as it is being replicated.
Ig分子由4条肽链组成,2条长链称为重链(heavychain,H),由大约440个氨基酸残基组成,分子量约50~70kD;2条短链称为轻链(lightchain,L),由大约220个氨基酸组成,分子量约22.5kD.4条肽链通过链间二硫键(-S·S-)连在一起。其结构模式见图2-1. Ig分子肽链的N端,在L链1/2和H链1/4处(约在110位前)氨基酸的种类和顺序各不相同,称为可变区(variableregion,V区);肽链C端其余部分的氨基酸,在种类和顺序上彼此间差别不大,称为
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